Application of RNA-seq technology in cancer chemoprevention

Research output: Chapter in Book/Report/Conference proceedingChapter


RNA-sequencing is a revolutionary tool to follow differential expression after treatment with cancer che-mopreventive agents. It allows a real genome-wide screening independent of prior assumptions and is well suited for analyzing coding but also long noncoding RNAs. It still consents the discovery of new genes and isoforms and increased our knowledge of antisense and other noncoding RNAs in a tremendous manner. Moreover, it permits to detect low-abundance and biologically critical isoforms and reveals genetic variants and gene fusions in one single assay. Here, we provide a detailed protocol for stranded RNA-sequencing.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages13
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
ISSN (Print)10643745


  • Chemoprevention
  • Deep-sequencing
  • Long noncoding RNA
  • Novel transcript and isoform discovery
  • RNA-Seq
  • Stranded RNA-Seq
  • Transcriptional signature
  • Transcriptome profiling

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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