Apyrase, the product of the virulence plasmid-encoded phoN2 (apy) gene of Shigella flexneri, is necessary for proper unipolar IcsA localization and for efficient intercellular spread

D. Santapaola, F. Del Chierico, A. Petrucca, S. Uzzau, M. Casalino, B. Colonna, R. Sessa, F. Berlutti, M. Nicoletti

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The role in virulence of the Shigella flexneri ospB-phoN2 operon has been evaluated. Here we confirm that OspB is an effector and show that apyrase, the product of phoN2, may be a virulence factor, since it is required for efficient intercellular spreading. Apyrase may be important in a deoxynucleoside triphosphate-hydrolyzing activity-independent manner, suggesting that it may act as an interaction partner in the process of IcsA localization.

Original languageEnglish
Pages (from-to)1620-1627
Number of pages8
JournalJournal of Bacteriology
Volume188
Issue number4
DOIs
Publication statusPublished - Feb 2006

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Apyrase
Shigella flexneri
Virulence
Plasmids
Virulence Factors
Operon
Genes
triphosphoric acid

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Apyrase, the product of the virulence plasmid-encoded phoN2 (apy) gene of Shigella flexneri, is necessary for proper unipolar IcsA localization and for efficient intercellular spread. / Santapaola, D.; Del Chierico, F.; Petrucca, A.; Uzzau, S.; Casalino, M.; Colonna, B.; Sessa, R.; Berlutti, F.; Nicoletti, M.

In: Journal of Bacteriology, Vol. 188, No. 4, 02.2006, p. 1620-1627.

Research output: Contribution to journalArticle

Santapaola, D. ; Del Chierico, F. ; Petrucca, A. ; Uzzau, S. ; Casalino, M. ; Colonna, B. ; Sessa, R. ; Berlutti, F. ; Nicoletti, M. / Apyrase, the product of the virulence plasmid-encoded phoN2 (apy) gene of Shigella flexneri, is necessary for proper unipolar IcsA localization and for efficient intercellular spread. In: Journal of Bacteriology. 2006 ; Vol. 188, No. 4. pp. 1620-1627.
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AU - Del Chierico, F.

AU - Petrucca, A.

AU - Uzzau, S.

AU - Casalino, M.

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AU - Sessa, R.

AU - Berlutti, F.

AU - Nicoletti, M.

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