AQP4-Dependent Water Transport Plays a Functional Role in Exercise-Induced Skeletal Muscle Adaptations

Davide Basco, Bert Blaauw, Francesco Pisani, Angelo Sparaneo, Grazia Paola Nicchia, Maria Grazia Mola, Carlo Reggiani, Maria Svelto, Antonio Frigeri

Research output: Contribution to journalArticle

Abstract

In this study we assess the functional role of Aquaporin-4 (AQP4) in the skeletal muscle by analyzing whether physical activity modulates AQP4 expression and whether the absence of AQP4 has an effect on osmotic behavior, muscle contractile properties, and physical activity. To this purpose, rats and mice were trained on the treadmill for 10 (D10) and 30 (D30) days and tested with exercise to exhaustion, and muscles were used for immunoblotting, RT-PCR, and fiber-type distribution analysis. Taking advantage of the AQP4 KO murine model, functional analysis of AQP4 was performed on dissected muscle fibers and sarcolemma vesicles. Moreover, WT and AQP4 KO mice were subjected to both voluntary and forced activity. Rat fast-twitch muscles showed a twofold increase in AQP4 protein in D10 and D30 rats compared to sedentary rats. Such increase positively correlated with the animal performance, since highest level of AQP4 protein was found in high runner rats. Interestingly, no shift in muscle fiber composition nor an increase in AQP4-positive fibers was found. Furthermore, no changes in AQP4 mRNA after exercise were detected, suggesting that post-translational events are likely to be responsible for AQP4 modulation. Experiments performed on AQP4 KO mice revealed a strong impairment in osmotic responses as well as in forced and voluntary activities compared to WT mice, even though force development amplitude and contractile properties were unvaried. Our findings definitively demonstrate the physiological role of AQP4 in supporting muscle contractile activity and metabolic changes that occur in fast-twitch skeletal muscle during prolonged exercise.

Original languageEnglish
Article numbere58712
JournalPLoS One
Volume8
Issue number3
DOIs
Publication statusPublished - Mar 8 2013

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Aquaporin 4
aquaporins
Muscle
skeletal muscle
Skeletal Muscle
exercise
Water
water
Rats
Muscles
rats
muscles
Fibers
mice
muscle fibers
physical activity
Exercise equipment
Sarcolemma
Functional analysis
exercise equipment

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

AQP4-Dependent Water Transport Plays a Functional Role in Exercise-Induced Skeletal Muscle Adaptations. / Basco, Davide; Blaauw, Bert; Pisani, Francesco; Sparaneo, Angelo; Nicchia, Grazia Paola; Mola, Maria Grazia; Reggiani, Carlo; Svelto, Maria; Frigeri, Antonio.

In: PLoS One, Vol. 8, No. 3, e58712, 08.03.2013.

Research output: Contribution to journalArticle

Basco, D, Blaauw, B, Pisani, F, Sparaneo, A, Nicchia, GP, Mola, MG, Reggiani, C, Svelto, M & Frigeri, A 2013, 'AQP4-Dependent Water Transport Plays a Functional Role in Exercise-Induced Skeletal Muscle Adaptations', PLoS One, vol. 8, no. 3, e58712. https://doi.org/10.1371/journal.pone.0058712
Basco, Davide ; Blaauw, Bert ; Pisani, Francesco ; Sparaneo, Angelo ; Nicchia, Grazia Paola ; Mola, Maria Grazia ; Reggiani, Carlo ; Svelto, Maria ; Frigeri, Antonio. / AQP4-Dependent Water Transport Plays a Functional Role in Exercise-Induced Skeletal Muscle Adaptations. In: PLoS One. 2013 ; Vol. 8, No. 3.
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