Abstract
Dendritic cells (DCs) are the most potent antigen-presenting cells able to trigger the adaptive immune response to specific antigens. When non-self-antigens are captured, DCs switch from an "immature" to a "mature" state to fulfill their function. Among the several surface proteins involved in DCs maturation, the role of aquaporins (AQPs) is still poorly understood. Here we investigated the expression profile of Aqps in murine bone marrow derived dendritic cells (BMDCs). Among the Aqps analyzed, Aqp9 was the most expressed by DCs. Its expression level was significantly upregulated 6 h following LPS exposure. Chemical inhibition of Aqp9 led to a decreased inflammatory cytokines secretion. BMDCs from AQP9-KO mice release lower amount of inflammatory cytokines and chemokines and increased release of IL-10. Despite the reduced release of inflammatory cytokines, Aqp9-KO mice were not protected from DSS induced colitis. All together, our data indicate that AQP9 blockade can be an efficient strategy to reduce DCs inflammatory response but it is not sufficient to protect from acute inflammatory insults such as DSS induced colitis.
| Original language | English |
|---|---|
| Pages (from-to) | 2355 |
| Number of pages | 9 |
| Journal | Frontiers in Immunology |
| Volume | 9 |
| DOIs | |
| Publication status | Published - 2018 |
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Aquaporin 9 Contributes to the Maturation Process and Inflammatory Cytokine Secretion of Murine Dendritic Cells. / De Santis, Stefania; Fiorentino, Maria R; Galleggiante, Vanessa; Verna, Giulio; Liso, Marina; Massaro, Monica; Lan, Jinggang; Troisi, Jacopo; Bertamino, Alessia; Pinto, Aldo; Campiglia, Pietro; Santino, Angelo; Giannelli, Gianluigi; Fasano, Alessio; Serino, Grazia; Chieppa, Marcello.
In: Frontiers in Immunology, Vol. 9, 2018, p. 2355.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Aquaporin 9 Contributes to the Maturation Process and Inflammatory Cytokine Secretion of Murine Dendritic Cells
AU - De Santis, Stefania
AU - Fiorentino, Maria R
AU - Galleggiante, Vanessa
AU - Verna, Giulio
AU - Liso, Marina
AU - Massaro, Monica
AU - Lan, Jinggang
AU - Troisi, Jacopo
AU - Bertamino, Alessia
AU - Pinto, Aldo
AU - Campiglia, Pietro
AU - Santino, Angelo
AU - Giannelli, Gianluigi
AU - Fasano, Alessio
AU - Serino, Grazia
AU - Chieppa, Marcello
PY - 2018
Y1 - 2018
N2 - Dendritic cells (DCs) are the most potent antigen-presenting cells able to trigger the adaptive immune response to specific antigens. When non-self-antigens are captured, DCs switch from an "immature" to a "mature" state to fulfill their function. Among the several surface proteins involved in DCs maturation, the role of aquaporins (AQPs) is still poorly understood. Here we investigated the expression profile of Aqps in murine bone marrow derived dendritic cells (BMDCs). Among the Aqps analyzed, Aqp9 was the most expressed by DCs. Its expression level was significantly upregulated 6 h following LPS exposure. Chemical inhibition of Aqp9 led to a decreased inflammatory cytokines secretion. BMDCs from AQP9-KO mice release lower amount of inflammatory cytokines and chemokines and increased release of IL-10. Despite the reduced release of inflammatory cytokines, Aqp9-KO mice were not protected from DSS induced colitis. All together, our data indicate that AQP9 blockade can be an efficient strategy to reduce DCs inflammatory response but it is not sufficient to protect from acute inflammatory insults such as DSS induced colitis.
AB - Dendritic cells (DCs) are the most potent antigen-presenting cells able to trigger the adaptive immune response to specific antigens. When non-self-antigens are captured, DCs switch from an "immature" to a "mature" state to fulfill their function. Among the several surface proteins involved in DCs maturation, the role of aquaporins (AQPs) is still poorly understood. Here we investigated the expression profile of Aqps in murine bone marrow derived dendritic cells (BMDCs). Among the Aqps analyzed, Aqp9 was the most expressed by DCs. Its expression level was significantly upregulated 6 h following LPS exposure. Chemical inhibition of Aqp9 led to a decreased inflammatory cytokines secretion. BMDCs from AQP9-KO mice release lower amount of inflammatory cytokines and chemokines and increased release of IL-10. Despite the reduced release of inflammatory cytokines, Aqp9-KO mice were not protected from DSS induced colitis. All together, our data indicate that AQP9 blockade can be an efficient strategy to reduce DCs inflammatory response but it is not sufficient to protect from acute inflammatory insults such as DSS induced colitis.
U2 - 10.3389/fimmu.2018.02355
DO - 10.3389/fimmu.2018.02355
M3 - Article
C2 - 30386332
VL - 9
SP - 2355
JO - Frontiers in Immunology
JF - Frontiers in Immunology
SN - 1664-3224
ER -