Aquaporin 9 Contributes to the Maturation Process and Inflammatory Cytokine Secretion of Murine Dendritic Cells

Stefania De Santis, Maria R Fiorentino, Vanessa Galleggiante, Giulio Verna, Marina Liso, Monica Massaro, Jinggang Lan, Jacopo Troisi, Alessia Bertamino, Aldo Pinto, Pietro Campiglia, Angelo Santino, Gianluigi Giannelli, Alessio Fasano, Grazia Serino, Marcello Chieppa

Research output: Contribution to journalArticle

Abstract

Dendritic cells (DCs) are the most potent antigen-presenting cells able to trigger the adaptive immune response to specific antigens. When non-self-antigens are captured, DCs switch from an "immature" to a "mature" state to fulfill their function. Among the several surface proteins involved in DCs maturation, the role of aquaporins (AQPs) is still poorly understood. Here we investigated the expression profile of Aqps in murine bone marrow derived dendritic cells (BMDCs). Among the Aqps analyzed, Aqp9 was the most expressed by DCs. Its expression level was significantly upregulated 6 h following LPS exposure. Chemical inhibition of Aqp9 led to a decreased inflammatory cytokines secretion. BMDCs from AQP9-KO mice release lower amount of inflammatory cytokines and chemokines and increased release of IL-10. Despite the reduced release of inflammatory cytokines, Aqp9-KO mice were not protected from DSS induced colitis. All together, our data indicate that AQP9 blockade can be an efficient strategy to reduce DCs inflammatory response but it is not sufficient to protect from acute inflammatory insults such as DSS induced colitis.

Original languageEnglish
Pages (from-to)2355
Number of pages9
JournalFrontiers in Immunology
Volume9
DOIs
Publication statusPublished - 2018

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Aquaporins
Dendritic Cells
Cytokines
Colitis
Bone Marrow
Antigens
Adaptive Immunity
Antigen-Presenting Cells
Chemokines
Interleukin-10
Membrane Proteins

Cite this

De Santis, S., Fiorentino, M. R., Galleggiante, V., Verna, G., Liso, M., Massaro, M., ... Chieppa, M. (2018). Aquaporin 9 Contributes to the Maturation Process and Inflammatory Cytokine Secretion of Murine Dendritic Cells. Frontiers in Immunology, 9, 2355. https://doi.org/10.3389/fimmu.2018.02355

Aquaporin 9 Contributes to the Maturation Process and Inflammatory Cytokine Secretion of Murine Dendritic Cells. / De Santis, Stefania; Fiorentino, Maria R; Galleggiante, Vanessa; Verna, Giulio; Liso, Marina; Massaro, Monica; Lan, Jinggang; Troisi, Jacopo; Bertamino, Alessia; Pinto, Aldo; Campiglia, Pietro; Santino, Angelo; Giannelli, Gianluigi; Fasano, Alessio; Serino, Grazia; Chieppa, Marcello.

In: Frontiers in Immunology, Vol. 9, 2018, p. 2355.

Research output: Contribution to journalArticle

De Santis, S, Fiorentino, MR, Galleggiante, V, Verna, G, Liso, M, Massaro, M, Lan, J, Troisi, J, Bertamino, A, Pinto, A, Campiglia, P, Santino, A, Giannelli, G, Fasano, A, Serino, G & Chieppa, M 2018, 'Aquaporin 9 Contributes to the Maturation Process and Inflammatory Cytokine Secretion of Murine Dendritic Cells', Frontiers in Immunology, vol. 9, pp. 2355. https://doi.org/10.3389/fimmu.2018.02355
De Santis, Stefania ; Fiorentino, Maria R ; Galleggiante, Vanessa ; Verna, Giulio ; Liso, Marina ; Massaro, Monica ; Lan, Jinggang ; Troisi, Jacopo ; Bertamino, Alessia ; Pinto, Aldo ; Campiglia, Pietro ; Santino, Angelo ; Giannelli, Gianluigi ; Fasano, Alessio ; Serino, Grazia ; Chieppa, Marcello. / Aquaporin 9 Contributes to the Maturation Process and Inflammatory Cytokine Secretion of Murine Dendritic Cells. In: Frontiers in Immunology. 2018 ; Vol. 9. pp. 2355.
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AU - De Santis, Stefania

AU - Fiorentino, Maria R

AU - Galleggiante, Vanessa

AU - Verna, Giulio

AU - Liso, Marina

AU - Massaro, Monica

AU - Lan, Jinggang

AU - Troisi, Jacopo

AU - Bertamino, Alessia

AU - Pinto, Aldo

AU - Campiglia, Pietro

AU - Santino, Angelo

AU - Giannelli, Gianluigi

AU - Fasano, Alessio

AU - Serino, Grazia

AU - Chieppa, Marcello

PY - 2018

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N2 - Dendritic cells (DCs) are the most potent antigen-presenting cells able to trigger the adaptive immune response to specific antigens. When non-self-antigens are captured, DCs switch from an "immature" to a "mature" state to fulfill their function. Among the several surface proteins involved in DCs maturation, the role of aquaporins (AQPs) is still poorly understood. Here we investigated the expression profile of Aqps in murine bone marrow derived dendritic cells (BMDCs). Among the Aqps analyzed, Aqp9 was the most expressed by DCs. Its expression level was significantly upregulated 6 h following LPS exposure. Chemical inhibition of Aqp9 led to a decreased inflammatory cytokines secretion. BMDCs from AQP9-KO mice release lower amount of inflammatory cytokines and chemokines and increased release of IL-10. Despite the reduced release of inflammatory cytokines, Aqp9-KO mice were not protected from DSS induced colitis. All together, our data indicate that AQP9 blockade can be an efficient strategy to reduce DCs inflammatory response but it is not sufficient to protect from acute inflammatory insults such as DSS induced colitis.

AB - Dendritic cells (DCs) are the most potent antigen-presenting cells able to trigger the adaptive immune response to specific antigens. When non-self-antigens are captured, DCs switch from an "immature" to a "mature" state to fulfill their function. Among the several surface proteins involved in DCs maturation, the role of aquaporins (AQPs) is still poorly understood. Here we investigated the expression profile of Aqps in murine bone marrow derived dendritic cells (BMDCs). Among the Aqps analyzed, Aqp9 was the most expressed by DCs. Its expression level was significantly upregulated 6 h following LPS exposure. Chemical inhibition of Aqp9 led to a decreased inflammatory cytokines secretion. BMDCs from AQP9-KO mice release lower amount of inflammatory cytokines and chemokines and increased release of IL-10. Despite the reduced release of inflammatory cytokines, Aqp9-KO mice were not protected from DSS induced colitis. All together, our data indicate that AQP9 blockade can be an efficient strategy to reduce DCs inflammatory response but it is not sufficient to protect from acute inflammatory insults such as DSS induced colitis.

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