Aquaretic inhibits renal cancer proliferation: Role of vasopressin receptor-2 (V2-R)

Davide Bolignano, Maria Antonietta Medici, Giuseppe Coppolino, Maria Teresa Sciortino, Francesca Marino Merlo, Susanna Campo, Valentina Donato, Assunta Venuti, Alessio Sturiale, Daniela Zaccaria, Antoine Buemi, Antonio Lacquaniti, Michele Buemi

Research output: Contribution to journalArticlepeer-review

Abstract

Vasopressin (AVP) is a hormone with antidiuretic properties that is also involved in cellular proliferation of breast, pulmonary, and pancreatic neoplasias, attributable to the interaction with specific receptors, among which is the V2-R. Using a culture model of CAKI-2 and A498 cancer cells, our study aimed to verify if renal carcinoma cells also express V2-R and whether receptor activation modulates their proliferation. Immunofluorescence and RT-PCR showed that both CAKI-2 and A498 cells effectively synthesize and express the V2-R. Administration of the vasopressin analogue DDAVP induced an evident growth in both CAKI-2 and A498 cell lines. However, this proliferative effect was completely avoided by the preventive addition of the V2-R antagonist SR121463B (satavaptan). Our study shows for the first time that renal cancer may effectively synthesize and express the V2-R. Furthermore, AVP exerts in vitro a proliferative effect by acting on this receptor, as the selective V2-R blockage is able to completely prevent the cellular growth. A validation of these findings with in vivo models is required to ascertain if the eventual presence of V2-R could influence the aggressiveness of human renal neoplasias. From this point of view, a new, interesting therapeutical application of V2-R antagonists in the treatment of renal cancer could also be proposed, similar to that successfully described in the treatment of autosomal polycystic kidney disease (ADPKD).

Original languageEnglish
Pages (from-to)642-647
Number of pages6
JournalUrologic Oncology: Seminars and Original Investigations
Volume28
Issue number6
DOIs
Publication statusPublished - Nov 2010

Keywords

  • Cell proliferation
  • Renal cancer
  • V2-R antagonist
  • V2-receptor
  • Vasopressin

ASJC Scopus subject areas

  • Oncology
  • Urology

Fingerprint Dive into the research topics of 'Aquaretic inhibits renal cancer proliferation: Role of vasopressin receptor-2 (V2-R)'. Together they form a unique fingerprint.

Cite this