Arachidonic acid metabolism and pathophysiologic aspects of subarachnoid hemorrhage in rats

Paolo Gaetani, Fulvio Marzatico, Riccardo Rodriguez Y Baena, Lucia Pacchiarini, Teresa Viganò, Guido Grignani, Maria Teresa Crivellari, Gianni Benzi

Research output: Contribution to journalArticlepeer-review


We studied the ex vivo production of prostaglandin D2, prostaglandin E2, 6-ketoprostaglandin F, and leukotriene C4 in the brain tissue of rats subjected to experimental subarachnoid hemorrhage. The ex vivo method allows the study of arachidonic acid metabolites released from brain slices at different times after subarachnoid hemorrhage induction and reflects the residual capacity for arachidonic acid metabolism after the pathologic event The rats were sacrificed 30 minutes, 1 and 6 hours, and 2 days after subarachnoid hemorrhage was induced by the injection of 0.30 ml autologous arterial blood into the cisterna magna. Concentration of prostaglandin D2 and 6-ketoprostaglandin Fwas increased significantly relative to control 2 days after induction. The concentration of prostaglandin E2 was increased significantly 6 hours after induction, while ex vivo production of leukotriene C4 was increased significantly at 1 and 6 hours and 2 days. The correlation between these results and the occurrence of vasospasm after subarachnoid hemorrhage is discussed. The results obtained from the ex vivo incubation of brain tissue slices after experimental subarachnoid hemorrhage suggest that after the hemorrhage there is a significant modification of brain eicosanoid metabolism, which could be of great importance in interpreting the pathogenesis of subarachnoid hemorrhage-related neuronal impairment.

Original languageEnglish
Pages (from-to)328-332
Number of pages5
Issue number2
Publication statusPublished - 1990


  • Leukotrienes
  • Prostaglandins
  • Rats
  • Subarachnoid hemorrhage

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Advanced and Specialised Nursing
  • Neuroscience(all)


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