Archaeal protoglobin structure indicates new ligand diffusion paths and modulation of haem-reactivity

Marco Nardini, Alessandra Pesce, Liesbet Thijs, Jennifer A. Saito, Sylvia Dewilde, Maqsudul Alam, Paolo Ascenzi, Massimiliano Coletta, Chiara Ciaccio, Luc Moens, Martino Bolognesi

Research output: Contribution to journalArticle

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Abstract

The structural adaptability of the globin fold has been highlighted by the recent discovery of the 2-on-2 haemoglobins, of neuroglobin and cytoglobin. Protoglobin from Methanosarcina acetivorans C2A-a strictly anaerobic methanogenic Archaea-is, to the best of our knowledge, the latest entry adding new variability and functional complexity to the haemoglobin (Hb) superfamily. Here, we report the 1.3 Å crystal structure of oxygenated M. acetivorans protoglobin, together with the first insight into its ligand-binding properties. We show that, contrary to all known globins, protoglobin-specific loops and an amino-terminal extension completely bury the haem within the protein matrix. Access of O2, CO and NO to the haem is granted by the protoglobin-specific apolar tunnels reaching the haem distal site from locations at the B/G and B/E helix interfaces. Functionally, M. acetivorans dimeric protoglobin shows a selectivity ratio for O2/CO binding to the haem that favours O2 ligation and anticooperativity in ligand binding. Both properties are exceptional within the Hb superfamily.

Original languageEnglish
Pages (from-to)157-163
Number of pages7
JournalEMBO Reports
Volume9
Issue number2
DOIs
Publication statusPublished - Feb 2008

Fingerprint

Heme
Modulation
Ligands
Hemoglobins
Globins
Carbon Monoxide
Methanosarcina
Archaea
Ligation
Tunnels
Crystal structure
Proteins

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Nardini, M., Pesce, A., Thijs, L., Saito, J. A., Dewilde, S., Alam, M., ... Bolognesi, M. (2008). Archaeal protoglobin structure indicates new ligand diffusion paths and modulation of haem-reactivity. EMBO Reports, 9(2), 157-163. https://doi.org/10.1038/sj.embor.7401153

Archaeal protoglobin structure indicates new ligand diffusion paths and modulation of haem-reactivity. / Nardini, Marco; Pesce, Alessandra; Thijs, Liesbet; Saito, Jennifer A.; Dewilde, Sylvia; Alam, Maqsudul; Ascenzi, Paolo; Coletta, Massimiliano; Ciaccio, Chiara; Moens, Luc; Bolognesi, Martino.

In: EMBO Reports, Vol. 9, No. 2, 02.2008, p. 157-163.

Research output: Contribution to journalArticle

Nardini, M, Pesce, A, Thijs, L, Saito, JA, Dewilde, S, Alam, M, Ascenzi, P, Coletta, M, Ciaccio, C, Moens, L & Bolognesi, M 2008, 'Archaeal protoglobin structure indicates new ligand diffusion paths and modulation of haem-reactivity', EMBO Reports, vol. 9, no. 2, pp. 157-163. https://doi.org/10.1038/sj.embor.7401153
Nardini, Marco ; Pesce, Alessandra ; Thijs, Liesbet ; Saito, Jennifer A. ; Dewilde, Sylvia ; Alam, Maqsudul ; Ascenzi, Paolo ; Coletta, Massimiliano ; Ciaccio, Chiara ; Moens, Luc ; Bolognesi, Martino. / Archaeal protoglobin structure indicates new ligand diffusion paths and modulation of haem-reactivity. In: EMBO Reports. 2008 ; Vol. 9, No. 2. pp. 157-163.
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