Are HLA class II and immunoglobulin constant region genes involved in the pathogenesis of mixed cryoglobulinemia type II after hepatitis C virus infection?

Antonio Amoroso, Monica Berrino, Lorena Canale, Maura Cornaglia, Simonetta Guarrera, Gina Mazzola, Silvana Savoldi, Francesco Scolari, Matti Sällberg, Massimo Clementi, Armando Gabrielli

Research output: Contribution to journalArticle

Abstract

Background/Aims: Hepatitis C virus infection is known to play an important role in the pathogenesis of essential mixed cryoglobulinemia type II. Progression of hepatitis C virus infection to mixed cryoglobulinemia may be influenced by host immune response. To analyze the immunogenetic background of mixed cryoglobulinemia, we studied HLA-DR, DQ loci and the switch regions of immunoglobulin heavy chain γ1 and γ4 constant genes. Methods: HLA typing was performed in 84 hepatitis C virus-infected patients (46 with cryoglobulins and 38 without), and 109 healthy controls, through analysis of restriction fragment length polymorphisms, supplemented with other techniques. Immunoglobulin heavy chain γ1 and γ4 polymorphisms, detected by restriction fragment length polymorphisms, were studied in 41 patients with mixed cryoglobulinemia and 51 controls. Results: The gene frequency of DRB1*11 was significantly higher in patients with mixed cryoglobulinemia than in controls (0.36 and 0.20, respectively; p= 0.0035). However, DRB1*11 was also increased in the subgroup of patients without mixed cryoglobulinemia who did not develop severe liver disease, while it was decreased in those with severe liver damage (0.50 and 0.13; p=0.0035). The frequency of 5.4 kb allele of the immunoglobulin heavy chain γ1 switch region was higher in patients with mixed cryoglobulinemia than in controls (0.47 and 0.22; p(c)=0.002), while the frequency of 5.5 kh allele was lower (0.51 and 0.78; p(c)= 0.001). Conclusions: Susceptibility to develop cryoglobulins after hepatitis C virus infection was not associated with HLA- DR or DQ. HLA-DRB1*11-positive individuals were protected from serious chronic liver disease after hepatitis C virus infection. Immunoglobulin heavy chain constant γ1 switch region restriction fragment length polymorphisms were associated with mixed cryoglobulinemia.

Original languageEnglish
Pages (from-to)36-44
Number of pages9
JournalJournal of Hepatology
Volume29
Issue number1
DOIs
Publication statusPublished - Jul 1998

Keywords

  • Genetics
  • Hepatitis C virus
  • HLA
  • IGHC
  • Immunogenetics
  • Mixed cryoglobulinemia

ASJC Scopus subject areas

  • Gastroenterology

Fingerprint Dive into the research topics of 'Are HLA class II and immunoglobulin constant region genes involved in the pathogenesis of mixed cryoglobulinemia type II after hepatitis C virus infection?'. Together they form a unique fingerprint.

  • Cite this

    Amoroso, A., Berrino, M., Canale, L., Cornaglia, M., Guarrera, S., Mazzola, G., Savoldi, S., Scolari, F., Sällberg, M., Clementi, M., & Gabrielli, A. (1998). Are HLA class II and immunoglobulin constant region genes involved in the pathogenesis of mixed cryoglobulinemia type II after hepatitis C virus infection? Journal of Hepatology, 29(1), 36-44. https://doi.org/10.1016/S0168-8278(98)80176-1