Are three generations of quantitative molecular methods sufficient in medical virology?

Massimo Clementi, Patrizia Bagnarelli

Research output: Contribution to journalArticle

Abstract

In the last two decades, development of quantitative molecular methods has characterized the evolution of clinical virology more than any other methodological advancement. Using these methods, a great deal of studies has addressed efficiently in vivo the role of viral load, viral replication activity, and viral transcriptional profiles as correlates of disease outcome and progression, and has highlighted the physio-pathology of important virus diseases of humans. Furthermore, these studies have contributed to a better understanding of virus-host interactions and have sharply revolutionized the research strategies in basic and medical virology. In addition and importantly from a medical point of view, quantitative methods have provided a rationale for the therapeutic intervention and therapy monitoring in medically important viral diseases. Despite the advances in technology and the development of three generations of molecular methods within the last two decades (competitive PCR, real-time PCR, and digital PCR), great challenges still remain for viral testing related not only to standardization, accuracy, and precision, but also to selection of the best molecular targets for clinical use and to the identification of thresholds for risk stratification and therapeutic decisions. Future research directions, novel methods and technical improvements could be important to address these challenges.

Original languageEnglish
Pages (from-to)437-441
Number of pages5
JournalNew Microbiologica
Volume38
Issue number4
Publication statusPublished - Oct 1 2015

Fingerprint

Virology
Virus Diseases
Polymerase Chain Reaction
Viral Load
Disease Progression
Real-Time Polymerase Chain Reaction
Therapeutics
Pathology
Viruses
Technology
Research

Keywords

  • Competitive PCR
  • Digital PCR
  • Quantitative methods
  • Real-time PCR
  • Viral load

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Are three generations of quantitative molecular methods sufficient in medical virology? / Clementi, Massimo; Bagnarelli, Patrizia.

In: New Microbiologica, Vol. 38, No. 4, 01.10.2015, p. 437-441.

Research output: Contribution to journalArticle

@article{82198ce61fea46c994feb1f652372236,
title = "Are three generations of quantitative molecular methods sufficient in medical virology?",
abstract = "In the last two decades, development of quantitative molecular methods has characterized the evolution of clinical virology more than any other methodological advancement. Using these methods, a great deal of studies has addressed efficiently in vivo the role of viral load, viral replication activity, and viral transcriptional profiles as correlates of disease outcome and progression, and has highlighted the physio-pathology of important virus diseases of humans. Furthermore, these studies have contributed to a better understanding of virus-host interactions and have sharply revolutionized the research strategies in basic and medical virology. In addition and importantly from a medical point of view, quantitative methods have provided a rationale for the therapeutic intervention and therapy monitoring in medically important viral diseases. Despite the advances in technology and the development of three generations of molecular methods within the last two decades (competitive PCR, real-time PCR, and digital PCR), great challenges still remain for viral testing related not only to standardization, accuracy, and precision, but also to selection of the best molecular targets for clinical use and to the identification of thresholds for risk stratification and therapeutic decisions. Future research directions, novel methods and technical improvements could be important to address these challenges.",
keywords = "Competitive PCR, Digital PCR, Quantitative methods, Real-time PCR, Viral load",
author = "Massimo Clementi and Patrizia Bagnarelli",
year = "2015",
month = "10",
day = "1",
language = "English",
volume = "38",
pages = "437--441",
journal = "New Microbiologica",
issn = "1121-7138",
publisher = "Luigi Ponzio e figlio Editori",
number = "4",

}

TY - JOUR

T1 - Are three generations of quantitative molecular methods sufficient in medical virology?

AU - Clementi, Massimo

AU - Bagnarelli, Patrizia

PY - 2015/10/1

Y1 - 2015/10/1

N2 - In the last two decades, development of quantitative molecular methods has characterized the evolution of clinical virology more than any other methodological advancement. Using these methods, a great deal of studies has addressed efficiently in vivo the role of viral load, viral replication activity, and viral transcriptional profiles as correlates of disease outcome and progression, and has highlighted the physio-pathology of important virus diseases of humans. Furthermore, these studies have contributed to a better understanding of virus-host interactions and have sharply revolutionized the research strategies in basic and medical virology. In addition and importantly from a medical point of view, quantitative methods have provided a rationale for the therapeutic intervention and therapy monitoring in medically important viral diseases. Despite the advances in technology and the development of three generations of molecular methods within the last two decades (competitive PCR, real-time PCR, and digital PCR), great challenges still remain for viral testing related not only to standardization, accuracy, and precision, but also to selection of the best molecular targets for clinical use and to the identification of thresholds for risk stratification and therapeutic decisions. Future research directions, novel methods and technical improvements could be important to address these challenges.

AB - In the last two decades, development of quantitative molecular methods has characterized the evolution of clinical virology more than any other methodological advancement. Using these methods, a great deal of studies has addressed efficiently in vivo the role of viral load, viral replication activity, and viral transcriptional profiles as correlates of disease outcome and progression, and has highlighted the physio-pathology of important virus diseases of humans. Furthermore, these studies have contributed to a better understanding of virus-host interactions and have sharply revolutionized the research strategies in basic and medical virology. In addition and importantly from a medical point of view, quantitative methods have provided a rationale for the therapeutic intervention and therapy monitoring in medically important viral diseases. Despite the advances in technology and the development of three generations of molecular methods within the last two decades (competitive PCR, real-time PCR, and digital PCR), great challenges still remain for viral testing related not only to standardization, accuracy, and precision, but also to selection of the best molecular targets for clinical use and to the identification of thresholds for risk stratification and therapeutic decisions. Future research directions, novel methods and technical improvements could be important to address these challenges.

KW - Competitive PCR

KW - Digital PCR

KW - Quantitative methods

KW - Real-time PCR

KW - Viral load

UR - http://www.scopus.com/inward/record.url?scp=84947944430&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84947944430&partnerID=8YFLogxK

M3 - Article

C2 - 26485007

AN - SCOPUS:84947944430

VL - 38

SP - 437

EP - 441

JO - New Microbiologica

JF - New Microbiologica

SN - 1121-7138

IS - 4

ER -