TY - JOUR
T1 - Arf, Arl, Arp and Sar proteins
T2 - A family of GTP-binding proteins with a structural device for 'front-back' communication
AU - Pasqualato, Sebastiano
AU - Renault, Louis
AU - Cherfils, Jacqueline
PY - 2002/11/1
Y1 - 2002/11/1
N2 - Arf proteins are important regulators of cellular traffic and the founding members of an expanding family of homologous proteins and genomic sequences. They depart from other small GTP-binding proteins by a unique structural device, which we call the 'interswitch toggle', that implements front-back communication from the N-terminus to the nucleotide binding site. Here we define the sequence and structural determinants that propagate information across the protein and identify them in all of the Arf family proteins other than Arl6 and Arl4/Arl7. The positions of these determinants lead us to propose that Arf family members with the interswitch toggle device are activated by a bipartite mechanism acting on opposite sides of the protein. The presence of this communication device might provide a more useful basis for unifying Arf homologs as a family than do the cellular functions of these proteins, which are mostly unrelated. We review available genomic sequences and functional data from this perspective, and identify a novel subfamily that we call Arl8.
AB - Arf proteins are important regulators of cellular traffic and the founding members of an expanding family of homologous proteins and genomic sequences. They depart from other small GTP-binding proteins by a unique structural device, which we call the 'interswitch toggle', that implements front-back communication from the N-terminus to the nucleotide binding site. Here we define the sequence and structural determinants that propagate information across the protein and identify them in all of the Arf family proteins other than Arl6 and Arl4/Arl7. The positions of these determinants lead us to propose that Arf family members with the interswitch toggle device are activated by a bipartite mechanism acting on opposite sides of the protein. The presence of this communication device might provide a more useful basis for unifying Arf homologs as a family than do the cellular functions of these proteins, which are mostly unrelated. We review available genomic sequences and functional data from this perspective, and identify a novel subfamily that we call Arl8.
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U2 - 10.1093/embo-reports/kvf221
DO - 10.1093/embo-reports/kvf221
M3 - Article
C2 - 12429613
AN - SCOPUS:0036866606
VL - 3
SP - 1035
EP - 1041
JO - EMBO Reports
JF - EMBO Reports
SN - 1469-221X
IS - 11
ER -