ARF mediates recruitment of Ptdlns-4-OH kinase-β and stimulates synthesis of Ptdlns(4,5)P2 on the Golgi complex

Anna Godi, Paolo Pertile, Rachel Meyers, Pierfrancesco Marra, Giuseppe Di Tullio, Cristiano Iurisci, Alberto Luini, Daniela Corda, Maria Antonietta De Matteis

Research output: Contribution to journalArticlepeer-review

Abstract

The small GTPase ADP-ribosylation factor (ARF) regulates the structure and function of the Golgi complex through mechanisms that are understood only in part, and which include an ability to control the assembly of coat complexes and phospholipase D (PLD). Here we describe a new property of ARF, the ability to recruit phosphatidylinositol-4-OH kinase-β and a still unidentified phosphatidylinositol-4-phosphate-5-OH kinase to the Golgi complex, resulting in a potent stimulation of synthesis of phosphatidylinositol-4-phosphate and phosphatidylinositol-4,5-bisphosphate; this ability is independent of its activities on coat proteins and PLD. Phosphatidylinositol-4-OH kinase-β is required for the structural integrity of the Golgi complex: transfection of a dominant-negative mutant of the kinase markedly alters the organization of the organelle.

Original languageEnglish
Pages (from-to)280-287
Number of pages8
JournalNature Cell Biology
Volume1
Issue number5
Publication statusPublished - Sep 1999

ASJC Scopus subject areas

  • Cell Biology

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