ARGONAUTE2 cooperates with SWI/SNF complex to determine nucleosome occupancy at human Transcription Start Sites

Claudia Carissimi, Ilaria Laudadio, Emanuela Cipolletta, Silvia Gioiosa, Marija Mihailovich, Tiziana Bonaldi, Giuseppe Macino, Valerio Fulci

Research output: Contribution to journalArticle

Abstract

Argonaute (AGO) proteins have a well-established role in post-transcriptional regulation of gene expression as key component of the RNA silencing pathways. Recent evidence involves AGO proteins in mammalian nuclear processes such as transcription and splicing, though the mechanistic aspects of AGO nuclear functions remain largely elusive. Here, by SILAC-based interaction proteomics, we identify the chromatin-remodelling complex SWI/SNF as a novel AGO2 interactor in human cells. Moreover, we show that nuclear AGO2 is loaded with a novel class of Dicer-dependent short RNAs (sRNAs), that we called swiRNAs, which map nearby the Transcription Start Sites (TSSs) bound by SWI/SNF. The knockdown of AGO2 decreases nucleosome occupancy at the first nucleosome located downstream of TSSs in a swiRNA-dependent manner. Our findings indicate that in human cells AGO2 binds SWI/SNF and a novel class of sRNAs to establish nucleosome occupancy on target TSSs.

Original languageEnglish
Pages (from-to)1498-1512
Number of pages15
JournalNucleic Acids Research
Volume43
Issue number3
DOIs
Publication statusPublished - 2015

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Nucleosomes
Transcription Initiation Site
Argonaute Proteins
RNA
Chromatin Assembly and Disassembly
Gene Expression Regulation
RNA Interference
Proteomics

ASJC Scopus subject areas

  • Genetics

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ARGONAUTE2 cooperates with SWI/SNF complex to determine nucleosome occupancy at human Transcription Start Sites. / Carissimi, Claudia; Laudadio, Ilaria; Cipolletta, Emanuela; Gioiosa, Silvia; Mihailovich, Marija; Bonaldi, Tiziana; Macino, Giuseppe; Fulci, Valerio.

In: Nucleic Acids Research, Vol. 43, No. 3, 2015, p. 1498-1512.

Research output: Contribution to journalArticle

Carissimi, Claudia ; Laudadio, Ilaria ; Cipolletta, Emanuela ; Gioiosa, Silvia ; Mihailovich, Marija ; Bonaldi, Tiziana ; Macino, Giuseppe ; Fulci, Valerio. / ARGONAUTE2 cooperates with SWI/SNF complex to determine nucleosome occupancy at human Transcription Start Sites. In: Nucleic Acids Research. 2015 ; Vol. 43, No. 3. pp. 1498-1512.
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