AROS-29 is involved in adaptive response to oxidative stress

Nicola Montesano Gesualdi, Giuseppa Chirico, Maria Teresa Catanese, Giuseppe Pirozzi, Franca Esposito

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Transient adaptation to mild oxidative stress was induced in human osteosarcoma cells chronically grown in sub-toxic concentrations of diethylmaleate (DEM), a glutathione (GSH) depleting agent. The adapted cells, compared to untreated cells, contain increased concentrations of GSH (4-6 fold) which, upon DEM withdrawal from the culture medium, return to normal values and are more resistant to subsequent oxidizing stress induced either by toxic concentrations of the same agent or by (H2O2) treatment. To investigate the molecular mechanisms involved in the adaptive response to oxidative stress, we analyzed the gene expression profiles of DEM-adapted cells by differential display. The expression of adaptive response to oxidative stress (AROS)-29 gene, coding for a transmembrane protein of unknown function, as well as of some known genes involved in energy metabolism, protein folding and membrane traffic is up-regulated in adapted cells. The increased resistance to both DNA damage and apoptosis, in cells stably overexpressing AROS-29, demonstrated its functional role in the protection against oxidative stress.

Original languageEnglish
Pages (from-to)467-476
Number of pages10
JournalFree Radical Research
Volume40
Issue number5
DOIs
Publication statusPublished - May 2006

Fingerprint

diethyl maleate
Oxidative stress
Oxidative Stress
Poisons
Genes
Protein folding
Gene expression
Protein Folding
Glutathione
Osteosarcoma
Culture Media
Transcriptome
Energy Metabolism
DNA Damage
Display devices
Apoptosis
Reference Values
Membranes
DNA
Proteins

Keywords

  • Adaptive responses
  • Diethylmaleate
  • Gene expression
  • Oxidative stress
  • Reactive oxygen Species

ASJC Scopus subject areas

  • Biochemistry

Cite this

Gesualdi, N. M., Chirico, G., Catanese, M. T., Pirozzi, G., & Esposito, F. (2006). AROS-29 is involved in adaptive response to oxidative stress. Free Radical Research, 40(5), 467-476. https://doi.org/10.1080/10715760600570547

AROS-29 is involved in adaptive response to oxidative stress. / Gesualdi, Nicola Montesano; Chirico, Giuseppa; Catanese, Maria Teresa; Pirozzi, Giuseppe; Esposito, Franca.

In: Free Radical Research, Vol. 40, No. 5, 05.2006, p. 467-476.

Research output: Contribution to journalArticle

Gesualdi, NM, Chirico, G, Catanese, MT, Pirozzi, G & Esposito, F 2006, 'AROS-29 is involved in adaptive response to oxidative stress', Free Radical Research, vol. 40, no. 5, pp. 467-476. https://doi.org/10.1080/10715760600570547
Gesualdi NM, Chirico G, Catanese MT, Pirozzi G, Esposito F. AROS-29 is involved in adaptive response to oxidative stress. Free Radical Research. 2006 May;40(5):467-476. https://doi.org/10.1080/10715760600570547
Gesualdi, Nicola Montesano ; Chirico, Giuseppa ; Catanese, Maria Teresa ; Pirozzi, Giuseppe ; Esposito, Franca. / AROS-29 is involved in adaptive response to oxidative stress. In: Free Radical Research. 2006 ; Vol. 40, No. 5. pp. 467-476.
@article{58afafae033e4e6d97335ee9bc14188b,
title = "AROS-29 is involved in adaptive response to oxidative stress",
abstract = "Transient adaptation to mild oxidative stress was induced in human osteosarcoma cells chronically grown in sub-toxic concentrations of diethylmaleate (DEM), a glutathione (GSH) depleting agent. The adapted cells, compared to untreated cells, contain increased concentrations of GSH (4-6 fold) which, upon DEM withdrawal from the culture medium, return to normal values and are more resistant to subsequent oxidizing stress induced either by toxic concentrations of the same agent or by (H2O2) treatment. To investigate the molecular mechanisms involved in the adaptive response to oxidative stress, we analyzed the gene expression profiles of DEM-adapted cells by differential display. The expression of adaptive response to oxidative stress (AROS)-29 gene, coding for a transmembrane protein of unknown function, as well as of some known genes involved in energy metabolism, protein folding and membrane traffic is up-regulated in adapted cells. The increased resistance to both DNA damage and apoptosis, in cells stably overexpressing AROS-29, demonstrated its functional role in the protection against oxidative stress.",
keywords = "Adaptive responses, Diethylmaleate, Gene expression, Oxidative stress, Reactive oxygen Species",
author = "Gesualdi, {Nicola Montesano} and Giuseppa Chirico and Catanese, {Maria Teresa} and Giuseppe Pirozzi and Franca Esposito",
year = "2006",
month = "5",
doi = "10.1080/10715760600570547",
language = "English",
volume = "40",
pages = "467--476",
journal = "Free Radical Research",
issn = "1071-5762",
publisher = "Informa Healthcare",
number = "5",

}

TY - JOUR

T1 - AROS-29 is involved in adaptive response to oxidative stress

AU - Gesualdi, Nicola Montesano

AU - Chirico, Giuseppa

AU - Catanese, Maria Teresa

AU - Pirozzi, Giuseppe

AU - Esposito, Franca

PY - 2006/5

Y1 - 2006/5

N2 - Transient adaptation to mild oxidative stress was induced in human osteosarcoma cells chronically grown in sub-toxic concentrations of diethylmaleate (DEM), a glutathione (GSH) depleting agent. The adapted cells, compared to untreated cells, contain increased concentrations of GSH (4-6 fold) which, upon DEM withdrawal from the culture medium, return to normal values and are more resistant to subsequent oxidizing stress induced either by toxic concentrations of the same agent or by (H2O2) treatment. To investigate the molecular mechanisms involved in the adaptive response to oxidative stress, we analyzed the gene expression profiles of DEM-adapted cells by differential display. The expression of adaptive response to oxidative stress (AROS)-29 gene, coding for a transmembrane protein of unknown function, as well as of some known genes involved in energy metabolism, protein folding and membrane traffic is up-regulated in adapted cells. The increased resistance to both DNA damage and apoptosis, in cells stably overexpressing AROS-29, demonstrated its functional role in the protection against oxidative stress.

AB - Transient adaptation to mild oxidative stress was induced in human osteosarcoma cells chronically grown in sub-toxic concentrations of diethylmaleate (DEM), a glutathione (GSH) depleting agent. The adapted cells, compared to untreated cells, contain increased concentrations of GSH (4-6 fold) which, upon DEM withdrawal from the culture medium, return to normal values and are more resistant to subsequent oxidizing stress induced either by toxic concentrations of the same agent or by (H2O2) treatment. To investigate the molecular mechanisms involved in the adaptive response to oxidative stress, we analyzed the gene expression profiles of DEM-adapted cells by differential display. The expression of adaptive response to oxidative stress (AROS)-29 gene, coding for a transmembrane protein of unknown function, as well as of some known genes involved in energy metabolism, protein folding and membrane traffic is up-regulated in adapted cells. The increased resistance to both DNA damage and apoptosis, in cells stably overexpressing AROS-29, demonstrated its functional role in the protection against oxidative stress.

KW - Adaptive responses

KW - Diethylmaleate

KW - Gene expression

KW - Oxidative stress

KW - Reactive oxygen Species

UR - http://www.scopus.com/inward/record.url?scp=33645101644&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645101644&partnerID=8YFLogxK

U2 - 10.1080/10715760600570547

DO - 10.1080/10715760600570547

M3 - Article

C2 - 16551573

AN - SCOPUS:33645101644

VL - 40

SP - 467

EP - 476

JO - Free Radical Research

JF - Free Radical Research

SN - 1071-5762

IS - 5

ER -