Abstract
Transient adaptation to mild oxidative stress was induced in human osteosarcoma cells chronically grown in sub-toxic concentrations of diethylmaleate (DEM), a glutathione (GSH) depleting agent. The adapted cells, compared to untreated cells, contain increased concentrations of GSH (4-6 fold) which, upon DEM withdrawal from the culture medium, return to normal values and are more resistant to subsequent oxidizing stress induced either by toxic concentrations of the same agent or by (H2O2) treatment. To investigate the molecular mechanisms involved in the adaptive response to oxidative stress, we analyzed the gene expression profiles of DEM-adapted cells by differential display. The expression of adaptive response to oxidative stress (AROS)-29 gene, coding for a transmembrane protein of unknown function, as well as of some known genes involved in energy metabolism, protein folding and membrane traffic is up-regulated in adapted cells. The increased resistance to both DNA damage and apoptosis, in cells stably overexpressing AROS-29, demonstrated its functional role in the protection against oxidative stress.
| Original language | English |
|---|---|
| Pages (from-to) | 467-476 |
| Number of pages | 10 |
| Journal | Free Radical Research |
| Volume | 40 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 2006 |
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Keywords
- Adaptive responses
- Diethylmaleate
- Gene expression
- Oxidative stress
- Reactive oxygen Species
ASJC Scopus subject areas
- Biochemistry
Cite this
AROS-29 is involved in adaptive response to oxidative stress. / Gesualdi, Nicola Montesano; Chirico, Giuseppa; Catanese, Maria Teresa; Pirozzi, Giuseppe; Esposito, Franca.
In: Free Radical Research, Vol. 40, No. 5, 05.2006, p. 467-476.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - AROS-29 is involved in adaptive response to oxidative stress
AU - Gesualdi, Nicola Montesano
AU - Chirico, Giuseppa
AU - Catanese, Maria Teresa
AU - Pirozzi, Giuseppe
AU - Esposito, Franca
PY - 2006/5
Y1 - 2006/5
N2 - Transient adaptation to mild oxidative stress was induced in human osteosarcoma cells chronically grown in sub-toxic concentrations of diethylmaleate (DEM), a glutathione (GSH) depleting agent. The adapted cells, compared to untreated cells, contain increased concentrations of GSH (4-6 fold) which, upon DEM withdrawal from the culture medium, return to normal values and are more resistant to subsequent oxidizing stress induced either by toxic concentrations of the same agent or by (H2O2) treatment. To investigate the molecular mechanisms involved in the adaptive response to oxidative stress, we analyzed the gene expression profiles of DEM-adapted cells by differential display. The expression of adaptive response to oxidative stress (AROS)-29 gene, coding for a transmembrane protein of unknown function, as well as of some known genes involved in energy metabolism, protein folding and membrane traffic is up-regulated in adapted cells. The increased resistance to both DNA damage and apoptosis, in cells stably overexpressing AROS-29, demonstrated its functional role in the protection against oxidative stress.
AB - Transient adaptation to mild oxidative stress was induced in human osteosarcoma cells chronically grown in sub-toxic concentrations of diethylmaleate (DEM), a glutathione (GSH) depleting agent. The adapted cells, compared to untreated cells, contain increased concentrations of GSH (4-6 fold) which, upon DEM withdrawal from the culture medium, return to normal values and are more resistant to subsequent oxidizing stress induced either by toxic concentrations of the same agent or by (H2O2) treatment. To investigate the molecular mechanisms involved in the adaptive response to oxidative stress, we analyzed the gene expression profiles of DEM-adapted cells by differential display. The expression of adaptive response to oxidative stress (AROS)-29 gene, coding for a transmembrane protein of unknown function, as well as of some known genes involved in energy metabolism, protein folding and membrane traffic is up-regulated in adapted cells. The increased resistance to both DNA damage and apoptosis, in cells stably overexpressing AROS-29, demonstrated its functional role in the protection against oxidative stress.
KW - Adaptive responses
KW - Diethylmaleate
KW - Gene expression
KW - Oxidative stress
KW - Reactive oxygen Species
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UR - http://www.scopus.com/inward/citedby.url?scp=33645101644&partnerID=8YFLogxK
U2 - 10.1080/10715760600570547
DO - 10.1080/10715760600570547
M3 - Article
C2 - 16551573
AN - SCOPUS:33645101644
VL - 40
SP - 467
EP - 476
JO - Free Radical Research
JF - Free Radical Research
SN - 1071-5762
IS - 5
ER -