AROS-29 is involved in adaptive response to oxidative stress

Nicola Montesano Gesualdi, Giuseppa Chirico, Maria Teresa Catanese, Giuseppe Pirozzi, Franca Esposito

Research output: Contribution to journalArticle

Abstract

Transient adaptation to mild oxidative stress was induced in human osteosarcoma cells chronically grown in sub-toxic concentrations of diethylmaleate (DEM), a glutathione (GSH) depleting agent. The adapted cells, compared to untreated cells, contain increased concentrations of GSH (4-6 fold) which, upon DEM withdrawal from the culture medium, return to normal values and are more resistant to subsequent oxidizing stress induced either by toxic concentrations of the same agent or by (H2O2) treatment. To investigate the molecular mechanisms involved in the adaptive response to oxidative stress, we analyzed the gene expression profiles of DEM-adapted cells by differential display. The expression of adaptive response to oxidative stress (AROS)-29 gene, coding for a transmembrane protein of unknown function, as well as of some known genes involved in energy metabolism, protein folding and membrane traffic is up-regulated in adapted cells. The increased resistance to both DNA damage and apoptosis, in cells stably overexpressing AROS-29, demonstrated its functional role in the protection against oxidative stress.

Original languageEnglish
Pages (from-to)467-476
Number of pages10
JournalFree Radical Research
Volume40
Issue number5
DOIs
Publication statusPublished - May 2006

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Keywords

  • Adaptive responses
  • Diethylmaleate
  • Gene expression
  • Oxidative stress
  • Reactive oxygen Species

ASJC Scopus subject areas

  • Biochemistry

Cite this

Gesualdi, N. M., Chirico, G., Catanese, M. T., Pirozzi, G., & Esposito, F. (2006). AROS-29 is involved in adaptive response to oxidative stress. Free Radical Research, 40(5), 467-476. https://doi.org/10.1080/10715760600570547