TY - JOUR
T1 - Array-based comparative genomic hybridization in early-stage mycosis fungoides
T2 - Recurrent deletion of tumor suppressor genes BCL7A, SMAC/DIABLO, and RHOF
AU - Carbone, Angelo
AU - Bernardini, Laura
AU - Valenzano, Francesco
AU - Bottillo, Irene
AU - De Simone, Clara
AU - Capizzi, Rodolfo
AU - Capalbo, Anna
AU - Romano, Francesca
AU - Novelli, Antonio
AU - Dallapiccola, Bruno
AU - Amerio, Pierluigi
PY - 2008/12
Y1 - 2008/12
N2 - The etiology of mycosis fungoides (MF), the most frequent form of cutaneous T cell lymphoma (CTCL), is poorly understood. No specific genetic aberration has been detected, especially in early-stage disease, possibly due to the clinical and histological heterogeneity of patient series and to the different sources of malignant cells (skin, blood, or lymph node) included in most studies. Frozen skin biopsies from 16 patients with early-stage MF were studied using array-based comparative genomic hybridization. A DNA pool from healthy donors was used as the reference. Results demonstrated recurrent loss of 19, 7p22.1-p22.3, 7q11.1-q11.23, 9q34.12, 12q24.31, and 16q22.3-q23.1, and gain of 8q22.3-q23.1 and 21q22.12. The 12q24.31 region was recurrently deleted in 7/16 patients. Real-time PCR investigation for deletion of genes BCL7A, SMAC/DIABLO, and RHOF - three tumor suppressor genes with a putative role in hematological malignancies - demonstrated that they were deleted in 9, 10, and 13 cases, respectively. The identified genomic alterations and individual genes could yield important insights into the early steps of MF pathogenesis.
AB - The etiology of mycosis fungoides (MF), the most frequent form of cutaneous T cell lymphoma (CTCL), is poorly understood. No specific genetic aberration has been detected, especially in early-stage disease, possibly due to the clinical and histological heterogeneity of patient series and to the different sources of malignant cells (skin, blood, or lymph node) included in most studies. Frozen skin biopsies from 16 patients with early-stage MF were studied using array-based comparative genomic hybridization. A DNA pool from healthy donors was used as the reference. Results demonstrated recurrent loss of 19, 7p22.1-p22.3, 7q11.1-q11.23, 9q34.12, 12q24.31, and 16q22.3-q23.1, and gain of 8q22.3-q23.1 and 21q22.12. The 12q24.31 region was recurrently deleted in 7/16 patients. Real-time PCR investigation for deletion of genes BCL7A, SMAC/DIABLO, and RHOF - three tumor suppressor genes with a putative role in hematological malignancies - demonstrated that they were deleted in 9, 10, and 13 cases, respectively. The identified genomic alterations and individual genes could yield important insights into the early steps of MF pathogenesis.
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U2 - 10.1002/gcc.20601
DO - 10.1002/gcc.20601
M3 - Article
C2 - 18663754
AN - SCOPUS:57149107372
VL - 47
SP - 1067
EP - 1075
JO - Genes Chromosomes and Cancer
JF - Genes Chromosomes and Cancer
SN - 1045-2257
IS - 12
ER -