Abstract
The present study investigated the ability of two neuroblastoma cell lines (SK-N-SH, with one copy of N-myc, and SK-N-BE(2), with over 150 copies of N-myc) to recycle ascorbate by quantifying semidehydroascorbate reductase and dehydroascorbate reductase activities. Both cell lines expressed dehydroascorbate activity (SK-N-SH 28.4 ± 9.8, SK-N-BE(2) 21.7 ± 5.2 nmol/min/mg protein). Intracellular semidehydroascorbate activity was present only in SK-N-BE(2) cells (4.7 ± 1.2 nmol/min/mg protein). Extracellular ascorbate was regenerated by semidehydroascorbate membrane activity, the activity of SK-N-BE(2) being twice that of SK-N-SH cells. The present data may explain the ability of the tumor to progress or regress through mechanisms involving both myc oncogene and apoptosis.
Original language | English |
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Pages (from-to) | 819-822 |
Number of pages | 4 |
Journal | Anticancer Research |
Volume | 18 |
Issue number | 2 A |
Publication status | Published - Mar 1998 |
Keywords
- Ascorbic acid
- N-myc
- Neuroblastoma
ASJC Scopus subject areas
- Cancer Research
- Oncology