Ascorbic acid recycling in N-myc amplified human neuroblastoma cells

Isabella Savini; Ida D'angelo; Margherita Annicchiarico-Petruzzelli; Lorenza Bellincampi; Gerry Melino; Luciana Avigliano

Ascorbic acid recycling in N-myc amplified human neuroblastoma cells

Isabella Savini, Ida D'angelo, Margherita Annicchiarico-Petruzzelli, Lorenza Bellincampi, Gerry Melino, Luciana Avigliano

Istituto Dermopatico dell'Immacolata , IDI

Research output: Contribution to journal › Article › peer-review

Abstract

The present study investigated the ability of two neuroblastoma cell lines (SK-N-SH, with one copy of N-myc, and SK-N-BE(2), with over 150 copies of N-myc) to recycle ascorbate by quantifying semidehydroascorbate reductase and dehydroascorbate reductase activities. Both cell lines expressed dehydroascorbate activity (SK-N-SH 28.4 ± 9.8, SK-N-BE(2) 21.7 ± 5.2 nmol/min/mg protein). Intracellular semidehydroascorbate activity was present only in SK-N-BE(2) cells (4.7 ± 1.2 nmol/min/mg protein). Extracellular ascorbate was regenerated by semidehydroascorbate membrane activity, the activity of SK-N-BE(2) being twice that of SK-N-SH cells. The present data may explain the ability of the tumor to progress or regress through mechanisms involving both myc oncogene and apoptosis.

Original language	English
Pages (from-to)	819-822
Number of pages	4
Journal	Anticancer Research
Volume	18
Issue number	2 A
Publication status	Published - Mar 1998

Keywords

Ascorbic acid
N-myc
Neuroblastoma

ASJC Scopus subject areas

Cancer Research
Oncology

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title = "Ascorbic acid recycling in N-myc amplified human neuroblastoma cells",

abstract = "The present study investigated the ability of two neuroblastoma cell lines (SK-N-SH, with one copy of N-myc, and SK-N-BE(2), with over 150 copies of N-myc) to recycle ascorbate by quantifying semidehydroascorbate reductase and dehydroascorbate reductase activities. Both cell lines expressed dehydroascorbate activity (SK-N-SH 28.4 ± 9.8, SK-N-BE(2) 21.7 ± 5.2 nmol/min/mg protein). Intracellular semidehydroascorbate activity was present only in SK-N-BE(2) cells (4.7 ± 1.2 nmol/min/mg protein). Extracellular ascorbate was regenerated by semidehydroascorbate membrane activity, the activity of SK-N-BE(2) being twice that of SK-N-SH cells. The present data may explain the ability of the tumor to progress or regress through mechanisms involving both myc oncogene and apoptosis.",

keywords = "Ascorbic acid, N-myc, Neuroblastoma",

author = "Isabella Savini and Ida D'angelo and Margherita Annicchiarico-Petruzzelli and Lorenza Bellincampi and Gerry Melino and Luciana Avigliano",

year = "1998",

month = mar,

language = "English",

volume = "18",

pages = "819--822",

journal = "Anticancer Research",

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publisher = "International Institute of Anticancer Research",

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TY - JOUR

T1 - Ascorbic acid recycling in N-myc amplified human neuroblastoma cells

AU - Savini, Isabella

AU - D'angelo, Ida

AU - Annicchiarico-Petruzzelli, Margherita

AU - Bellincampi, Lorenza

AU - Melino, Gerry

AU - Avigliano, Luciana

PY - 1998/3

Y1 - 1998/3

N2 - The present study investigated the ability of two neuroblastoma cell lines (SK-N-SH, with one copy of N-myc, and SK-N-BE(2), with over 150 copies of N-myc) to recycle ascorbate by quantifying semidehydroascorbate reductase and dehydroascorbate reductase activities. Both cell lines expressed dehydroascorbate activity (SK-N-SH 28.4 ± 9.8, SK-N-BE(2) 21.7 ± 5.2 nmol/min/mg protein). Intracellular semidehydroascorbate activity was present only in SK-N-BE(2) cells (4.7 ± 1.2 nmol/min/mg protein). Extracellular ascorbate was regenerated by semidehydroascorbate membrane activity, the activity of SK-N-BE(2) being twice that of SK-N-SH cells. The present data may explain the ability of the tumor to progress or regress through mechanisms involving both myc oncogene and apoptosis.

AB - The present study investigated the ability of two neuroblastoma cell lines (SK-N-SH, with one copy of N-myc, and SK-N-BE(2), with over 150 copies of N-myc) to recycle ascorbate by quantifying semidehydroascorbate reductase and dehydroascorbate reductase activities. Both cell lines expressed dehydroascorbate activity (SK-N-SH 28.4 ± 9.8, SK-N-BE(2) 21.7 ± 5.2 nmol/min/mg protein). Intracellular semidehydroascorbate activity was present only in SK-N-BE(2) cells (4.7 ± 1.2 nmol/min/mg protein). Extracellular ascorbate was regenerated by semidehydroascorbate membrane activity, the activity of SK-N-BE(2) being twice that of SK-N-SH cells. The present data may explain the ability of the tumor to progress or regress through mechanisms involving both myc oncogene and apoptosis.

KW - Ascorbic acid

KW - N-myc

KW - Neuroblastoma

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M3 - Article

C2 - 9615725

AN - SCOPUS:0031866132

VL - 18

SP - 819

EP - 822

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 2 A

ER -

Ascorbic acid recycling in N-myc amplified human neuroblastoma cells

Abstract

Keywords

ASJC Scopus subject areas

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