Ascorbic acid recycling in N-myc amplified human neuroblastoma cells

Isabella Savini, Ida D'angelo, Margherita Annicchiarico-Petruzzelli, Lorenza Bellincampi, Gerry Melino, Luciana Avigliano

Research output: Contribution to journalArticle

Abstract

The present study investigated the ability of two neuroblastoma cell lines (SK-N-SH, with one copy of N-myc, and SK-N-BE(2), with over 150 copies of N-myc) to recycle ascorbate by quantifying semidehydroascorbate reductase and dehydroascorbate reductase activities. Both cell lines expressed dehydroascorbate activity (SK-N-SH 28.4 ± 9.8, SK-N-BE(2) 21.7 ± 5.2 nmol/min/mg protein). Intracellular semidehydroascorbate activity was present only in SK-N-BE(2) cells (4.7 ± 1.2 nmol/min/mg protein). Extracellular ascorbate was regenerated by semidehydroascorbate membrane activity, the activity of SK-N-BE(2) being twice that of SK-N-SH cells. The present data may explain the ability of the tumor to progress or regress through mechanisms involving both myc oncogene and apoptosis.

Original languageEnglish
Pages (from-to)819-822
Number of pages4
JournalAnticancer Research
Volume18
Issue number2 A
Publication statusPublished - Mar 1998

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Keywords

  • Ascorbic acid
  • N-myc
  • Neuroblastoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Savini, I., D'angelo, I., Annicchiarico-Petruzzelli, M., Bellincampi, L., Melino, G., & Avigliano, L. (1998). Ascorbic acid recycling in N-myc amplified human neuroblastoma cells. Anticancer Research, 18(2 A), 819-822.