Ascorbic acid recycling in N-myc amplified human neuroblastoma cells

Isabella Savini, Ida D'angelo, Margherita Annicchiarico-Petruzzelli, Lorenza Bellincampi, Gerry Melino, Luciana Avigliano

Research output: Contribution to journalArticlepeer-review


The present study investigated the ability of two neuroblastoma cell lines (SK-N-SH, with one copy of N-myc, and SK-N-BE(2), with over 150 copies of N-myc) to recycle ascorbate by quantifying semidehydroascorbate reductase and dehydroascorbate reductase activities. Both cell lines expressed dehydroascorbate activity (SK-N-SH 28.4 ± 9.8, SK-N-BE(2) 21.7 ± 5.2 nmol/min/mg protein). Intracellular semidehydroascorbate activity was present only in SK-N-BE(2) cells (4.7 ± 1.2 nmol/min/mg protein). Extracellular ascorbate was regenerated by semidehydroascorbate membrane activity, the activity of SK-N-BE(2) being twice that of SK-N-SH cells. The present data may explain the ability of the tumor to progress or regress through mechanisms involving both myc oncogene and apoptosis.

Original languageEnglish
Pages (from-to)819-822
Number of pages4
JournalAnticancer Research
Issue number2 A
Publication statusPublished - Mar 1998


  • Ascorbic acid
  • N-myc
  • Neuroblastoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology


Dive into the research topics of 'Ascorbic acid recycling in N-myc amplified human neuroblastoma cells'. Together they form a unique fingerprint.

Cite this