Aspartate-induced changes in extracellular free calcium in 'in vitro' hippocampal slices of rats

M. G. Marciani, J. Louvel, U. Heinemann

Research output: Contribution to journalArticlepeer-review


Extracellular free calcium ([Ca2+]0) and neuronal activity were measured with double-barreled Ca2+-selective/reference microelectrodes in the CA1 field of rat hippocampal slices during stimulation of Schaffer collaterals and during iontophoretic application of the excitatory amino acid aspartate (Asp). Baseline [Ca2+]0 was 1.5 mM. Repetitive stimulation and aspartate application caused decreases in [Ca2+]0 (ΔCa), which were largest in the pyramidal cell layer. During iontophoretic application of GABA, aspartate often failed to induce significant alterations in [Ca2+]0. Mg2+ (9 mM) and Ni2+ (3 mM) when added to the perfusion medium reduced aspartate-dependent ΔCa by 15 and 70% respectively. In tetrodotoxin (10-6 M) containing Ringer the aspartate-induced ΔCa were about 10% smaller. These findings suggest that the decreases in [Ca2+]0 are mainly due to a postsynaptic Ca2+ entry through selective Ca2+ conductances.

Original languageEnglish
Pages (from-to)272-277
Number of pages6
JournalBrain Research
Issue number1
Publication statusPublished - Apr 22 1982


  • aspartate
  • extracellular free calcium
  • hippocampal slice
  • rat

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)


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