Aspartate-induced changes in extracellular free calcium in 'in vitro' hippocampal slices of rats

M. G. Marciani; J. Louvel; U. Heinemann

doi:10.1016/0006-8993(82)90796-X

Aspartate-induced changes in extracellular free calcium in 'in vitro' hippocampal slices of rats

M. G. Marciani, J. Louvel, U. Heinemann

Fondazione Santa Lucia

Research output: Contribution to journal › Article › peer-review

Abstract

Extracellular free calcium ([Ca²⁺]₀) and neuronal activity were measured with double-barreled Ca²⁺-selective/reference microelectrodes in the CA1 field of rat hippocampal slices during stimulation of Schaffer collaterals and during iontophoretic application of the excitatory amino acid aspartate (Asp). Baseline [Ca²⁺]₀ was 1.5 mM. Repetitive stimulation and aspartate application caused decreases in [Ca²⁺]₀ (ΔCa), which were largest in the pyramidal cell layer. During iontophoretic application of GABA, aspartate often failed to induce significant alterations in [Ca²⁺]₀. Mg²⁺ (9 mM) and Ni²⁺ (3 mM) when added to the perfusion medium reduced aspartate-dependent ΔCa by 15 and 70% respectively. In tetrodotoxin (10^-6 M) containing Ringer the aspartate-induced ΔCa were about 10% smaller. These findings suggest that the decreases in [Ca²⁺]₀ are mainly due to a postsynaptic Ca²⁺ entry through selective Ca²⁺ conductances.

Original language	English
Pages (from-to)	272-277
Number of pages	6
Journal	Brain Research
Volume	238
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(82)90796-X
Publication status	Published - Apr 22 1982

Keywords

aspartate
extracellular free calcium
hippocampal slice
rat

ASJC Scopus subject areas

Developmental Biology
Molecular Biology
Clinical Neurology
Neuroscience(all)

Access to Document

10.1016/0006-8993(82)90796-X

Cite this

@article{43edd07efb7746f0bdcb50193d8f43be,

title = "Aspartate-induced changes in extracellular free calcium in 'in vitro' hippocampal slices of rats",

abstract = "Extracellular free calcium ([Ca2+]0) and neuronal activity were measured with double-barreled Ca2+-selective/reference microelectrodes in the CA1 field of rat hippocampal slices during stimulation of Schaffer collaterals and during iontophoretic application of the excitatory amino acid aspartate (Asp). Baseline [Ca2+]0 was 1.5 mM. Repetitive stimulation and aspartate application caused decreases in [Ca2+]0 (ΔCa), which were largest in the pyramidal cell layer. During iontophoretic application of GABA, aspartate often failed to induce significant alterations in [Ca2+]0. Mg2+ (9 mM) and Ni2+ (3 mM) when added to the perfusion medium reduced aspartate-dependent ΔCa by 15 and 70% respectively. In tetrodotoxin (10-6 M) containing Ringer the aspartate-induced ΔCa were about 10% smaller. These findings suggest that the decreases in [Ca2+]0 are mainly due to a postsynaptic Ca2+ entry through selective Ca2+ conductances.",

keywords = "aspartate, extracellular free calcium, hippocampal slice, rat",

author = "Marciani, {M. G.} and J. Louvel and U. Heinemann",

year = "1982",

month = apr,

day = "22",

doi = "10.1016/0006-8993(82)90796-X",

language = "English",

volume = "238",

pages = "272--277",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Aspartate-induced changes in extracellular free calcium in 'in vitro' hippocampal slices of rats

AU - Marciani, M. G.

AU - Louvel, J.

AU - Heinemann, U.

PY - 1982/4/22

Y1 - 1982/4/22

N2 - Extracellular free calcium ([Ca2+]0) and neuronal activity were measured with double-barreled Ca2+-selective/reference microelectrodes in the CA1 field of rat hippocampal slices during stimulation of Schaffer collaterals and during iontophoretic application of the excitatory amino acid aspartate (Asp). Baseline [Ca2+]0 was 1.5 mM. Repetitive stimulation and aspartate application caused decreases in [Ca2+]0 (ΔCa), which were largest in the pyramidal cell layer. During iontophoretic application of GABA, aspartate often failed to induce significant alterations in [Ca2+]0. Mg2+ (9 mM) and Ni2+ (3 mM) when added to the perfusion medium reduced aspartate-dependent ΔCa by 15 and 70% respectively. In tetrodotoxin (10-6 M) containing Ringer the aspartate-induced ΔCa were about 10% smaller. These findings suggest that the decreases in [Ca2+]0 are mainly due to a postsynaptic Ca2+ entry through selective Ca2+ conductances.

AB - Extracellular free calcium ([Ca2+]0) and neuronal activity were measured with double-barreled Ca2+-selective/reference microelectrodes in the CA1 field of rat hippocampal slices during stimulation of Schaffer collaterals and during iontophoretic application of the excitatory amino acid aspartate (Asp). Baseline [Ca2+]0 was 1.5 mM. Repetitive stimulation and aspartate application caused decreases in [Ca2+]0 (ΔCa), which were largest in the pyramidal cell layer. During iontophoretic application of GABA, aspartate often failed to induce significant alterations in [Ca2+]0. Mg2+ (9 mM) and Ni2+ (3 mM) when added to the perfusion medium reduced aspartate-dependent ΔCa by 15 and 70% respectively. In tetrodotoxin (10-6 M) containing Ringer the aspartate-induced ΔCa were about 10% smaller. These findings suggest that the decreases in [Ca2+]0 are mainly due to a postsynaptic Ca2+ entry through selective Ca2+ conductances.

KW - aspartate

KW - extracellular free calcium

KW - hippocampal slice

KW - rat

UR - http://www.scopus.com/inward/record.url?scp=0020048728&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020048728&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(82)90796-X

DO - 10.1016/0006-8993(82)90796-X

M3 - Article

C2 - 6282396

AN - SCOPUS:0020048728

VL - 238

SP - 272

EP - 277

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -

Aspartate-induced changes in extracellular free calcium in 'in vitro' hippocampal slices of rats

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this