Aspects of gene regulation during the UPR in human cells

Cristina Benedetti, Marco Fabbri, Roberto Sitia, Andrea Cabibbo

Research output: Contribution to journalArticle

Abstract

The accumulation of unfolded proteins in the endoplasmic reticulum (ER) activates a signaling pathway known as the unfolded protein response (UPR), which leads to the transcriptional activation of factors involved in ER protein folding, to a transitory inhibition of protein synthesis and to an upregulation of the ER-associated degradation pathway. In order to identify new genes regulated during the UPR we have used an RNA fingerprinting technique to analyze the gene expression profiles in cells treated with DTT or tunicamycin, two strong UPR inducers. We isolated two novel transcripts upregulated by both treatments. The selective regulation of these genes during the UPR was confirmed in different cell lines and under various UPR-inducing conditions. These studies highlighted interesting aspects of the gene expression during the UPR, including a selective downregulation of members of the hsp70 family. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)530-536
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume278
Issue number3
DOIs
Publication statusPublished - Nov 30 2000

Keywords

  • Differential display
  • Endoplasmic reticulum
  • ER stress
  • Heat shock protein
  • Protein folding

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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