Assay for 5-hydroxylysine and L-lysine in human and rat urine and in bone by gas chromatography

D. Euli, L. Colombo, A. Bruno, E. Mussini

Research output: Contribution to journalArticlepeer-review


An accurate method for the determination of collagen to study its distribution and turn-over in different tissues is described. 5-Hydroxylysine (5Hylys) is an amino acid that is apparently present in no other protein except collagen and, as it is metabolised only to a minor degree compared with 4-hydroxyproline (4Hypro), it has been suggested as a better marker of the collagen metabolism. Interest in this amino acid has increased recently because the levels of 5Hylys in urine and in different tissues may offer a new basis for detecting pathologies of the collagen molecule. This paper describes a method for the quantitative determination of 5Hylys and lysine (Lys) by gas chromatography (GC) in human and rat urine and in rat bone. The limit of detection was 350 pmolml -1 for 5Hylys and 200 pmolml -1 for Lys for all the biological samples. This method therefore provides a complete view of the metabolism of this amino acid and of the tissue it comes from. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)373-379
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Issue number2
Publication statusPublished - Mar 19 1999


  • 5-Hydroxylysine
  • Lysine

ASJC Scopus subject areas

  • Chemistry(all)


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