Assay of FAAH activity

Monica Bari, Monica Feole, Mauro Maccarrone

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Fatty acid amide hydrolase (FAAH) is an intracellular enzyme responsible for the hydrolysis of endogenous anandamide (AEA), a reaction that terminates the biological effects of this lipid mediator. The final products of this reaction are arachidonic acid and ethanolamine. In the method described herein, FAAH activity is measured through the use of a radioactive substrate by quantification of reaction products, that is, [14C]-ethanolamine from [14C-ethanolamine]-AEA.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages131-136
Number of pages6
Volume1412
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1412
ISSN (Print)10643745

Keywords

  • AEA
  • Arachidonic acid
  • Bioactive lipids
  • Endocannabinoids
  • Enzyme
  • Ethanolamine
  • FAAH

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Assay of FAAH activity'. Together they form a unique fingerprint.

Cite this