Assay of NAPE-PLD activity

Filomena Fezza, Nicolina Mastrangelo, Mauro Maccarrone

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

N-acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a prominent enzyme involved in the biosynthesis of fatty acid amides (FAAs), a family of bioactive lipids including anandamide (AEA) as the prototypical member. Here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin-layer chromatography (TLC).

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages123-130
Number of pages8
Volume1412
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1412
ISSN (Print)10643745

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Keywords

  • Anandamide
  • Fatty acid amides
  • N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D
  • N-acyl-phosphatidylethanolamines
  • N-palmitoylethanolamine
  • Radioactive assay
  • Thin-layer chromatography

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Fezza, F., Mastrangelo, N., & Maccarrone, M. (2016). Assay of NAPE-PLD activity. In Methods in Molecular Biology (Vol. 1412, pp. 123-130). (Methods in Molecular Biology; Vol. 1412). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3539-0_13