Assay of NAPE-PLD activity

Filomena Fezza; Nicolina Mastrangelo; Mauro Maccarrone

doi:10.1007/978-1-4939-3539-0_13

Assay of NAPE-PLD activity

Filomena Fezza, Nicolina Mastrangelo, Mauro Maccarrone

Fondazione Santa Lucia

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

N-acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a prominent enzyme involved in the biosynthesis of fatty acid amides (FAAs), a family of bioactive lipids including anandamide (AEA) as the prototypical member. Here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin-layer chromatography (TLC).

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	123-130
Number of pages	8
Volume	1412
DOIs	https://doi.org/10.1007/978-1-4939-3539-0_13
Publication status	Published - 2016

Publication series

Name	Methods in Molecular Biology
Volume	1412
ISSN (Print)	10643745

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Keywords

Anandamide
Fatty acid amides
N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D
N-acyl-phosphatidylethanolamines
N-palmitoylethanolamine
Radioactive assay
Thin-layer chromatography

ASJC Scopus subject areas

Molecular Biology
Genetics

Cite this

Fezza, F., Mastrangelo, N., & Maccarrone, M. (2016). Assay of NAPE-PLD activity. In Methods in Molecular Biology (Vol. 1412, pp. 123-130). (Methods in Molecular Biology; Vol. 1412). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3539-0_13

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Access to Document

10.1007/978-1-4939-3539-0_13