Assay using succinyldithiocholin as substrate: The method of choice for the measurement of cholinesterase catalytic in serum to diagnose succinyldicholine sensitivity

Andrea Mosca, Roberto Bonora, Ferruccio Ceriotti, Carlo Franzini, Giuliana Lando, Maria Cristina Patrosso, Martina Zaninotto, Mauro Panteghini

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

No comparative information is available concerning the ability of various cholinesterase (ChE) methods to identify succinyldicholine-sensitive patients, purely on the basis of the enzyme activity recorded in serum. Here, we evaluated six different methods for the measurement of ChE activity; 131 subjects were subdivided according to ChE phenotype and, therefore, to succinyldicholine sensitivity. ChE phenotype was determined by measuring dibucaine and fluoride numbers. DNA analysis was also performed to confirm correlation between the phenotype classification used in the study and the ChE genotype. The tested methods were significantly different in their ability to discriminate between the subjects with and without succinyldicholine-sensitive phenotypes. The succinyldithiocholine/5,5′-dithio-bis(2-nitrobenzoate) (DTNB) method showed the highest accuracy (area under the receiver operating characteristic (ROC) curve 0.97) followed by the propionylthiocholine/DTNB method (area under the ROC curve 0.94). On the other hand, the two methods using butyrylthiocholine as substrate and that employing benzoylcholine showed limited clinical utility in discriminating subjects at risk of prolonged apnea (area under the ROC curve≤0.9). Using the succinyldithiocholine method, a value≤23 U/l was approximately five times as likely to occur in a sensitive individual as in a normal one.

Original languageEnglish
Pages (from-to)317-322
Number of pages6
JournalClinical Chemistry and Laboratory Medicine
Volume41
Issue number3
DOIs
Publication statusPublished - 2003

Fingerprint

Succinylcholine
Cholinesterases
Assays
Dithionitrobenzoic Acid
Substrates
Serum
ROC Curve
Phenotype
Benzoylcholine
Butyrylthiocholine
Dibucaine
Enzyme activity
Fluorides
Apnea
DNA
Genotype
Enzymes

Keywords

  • Apnoea
  • Butyrylcholinesterase
  • Cholinesterase inhibitors
  • Succinylcholine

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Assay using succinyldithiocholin as substrate : The method of choice for the measurement of cholinesterase catalytic in serum to diagnose succinyldicholine sensitivity. / Mosca, Andrea; Bonora, Roberto; Ceriotti, Ferruccio; Franzini, Carlo; Lando, Giuliana; Patrosso, Maria Cristina; Zaninotto, Martina; Panteghini, Mauro.

In: Clinical Chemistry and Laboratory Medicine, Vol. 41, No. 3, 2003, p. 317-322.

Research output: Contribution to journalArticle

Mosca, Andrea ; Bonora, Roberto ; Ceriotti, Ferruccio ; Franzini, Carlo ; Lando, Giuliana ; Patrosso, Maria Cristina ; Zaninotto, Martina ; Panteghini, Mauro. / Assay using succinyldithiocholin as substrate : The method of choice for the measurement of cholinesterase catalytic in serum to diagnose succinyldicholine sensitivity. In: Clinical Chemistry and Laboratory Medicine. 2003 ; Vol. 41, No. 3. pp. 317-322.
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