TY - JOUR
T1 - Assays for determination of HIV resistance to antiviral drugs
AU - Baldanti, Fausto
AU - Paolucci, Stefania
AU - Dossena, Luca
AU - Gerna, Giuseppe
PY - 2004/8
Y1 - 2004/8
N2 - Resistance to antiretroviral drugs is one of the major pitfalls of combined treatment of HIV infection. Thus, timely identification of drug resistant HIV strains and precise evaluation of the level of resistance to the different antiretroviral drugs are crucial in the management of treated patients. Phenotypic determination of antiretroviral drug resistance evaluates the ability of an HIV strain to replicate in the presence of drug(s). Thus, this assay (either conventional or recombinant) provides a direct estimate of drug susceptibility. However, it is relatively difficult to perform and requires dedicated facilities. Thus, its use is still restricted. Genotypic determination of drug resistance is based upon detection of specific mutations in HIV genes encoding target enzymes or receptors. The assay provides an indirect evidence of drug susceptibility. However, it is easy to perform and does not require dedicated facilities. Thus, it is widely utilized in clinical practice. Its major limitation concerns the complexity of results interpretation which still awaits a general consensus.
AB - Resistance to antiretroviral drugs is one of the major pitfalls of combined treatment of HIV infection. Thus, timely identification of drug resistant HIV strains and precise evaluation of the level of resistance to the different antiretroviral drugs are crucial in the management of treated patients. Phenotypic determination of antiretroviral drug resistance evaluates the ability of an HIV strain to replicate in the presence of drug(s). Thus, this assay (either conventional or recombinant) provides a direct estimate of drug susceptibility. However, it is relatively difficult to perform and requires dedicated facilities. Thus, its use is still restricted. Genotypic determination of drug resistance is based upon detection of specific mutations in HIV genes encoding target enzymes or receptors. The assay provides an indirect evidence of drug susceptibility. However, it is easy to perform and does not require dedicated facilities. Thus, it is widely utilized in clinical practice. Its major limitation concerns the complexity of results interpretation which still awaits a general consensus.
UR - http://www.scopus.com/inward/record.url?scp=3242876664&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3242876664&partnerID=8YFLogxK
U2 - 10.2174/1389200043335496
DO - 10.2174/1389200043335496
M3 - Article
C2 - 15320703
AN - SCOPUS:3242876664
VL - 5
SP - 317
EP - 319
JO - Current Drug Metabolism
JF - Current Drug Metabolism
SN - 1389-2002
IS - 4
ER -