Assays for Membrane and Intracellular Signalling Events

Vittorio Tomasi, Cristiana Griffoni, Spartaco Santi, Patrizia Lenaz, Rosa Anna Iorio, Antonio Strillacci, Enzo Spisni

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The concept that cell signalling is topologically restricted and vectorially orientated has stimulated much work. Restriction has been explained by the identification of signalling protein complexes in membrane microdomains (rafts and caveolae). The participation of endothelial cell caveolae to angiogenesis has been well reported and a distinction between normal and tumour-induced angiogenesis has been clearly documented. These advancements in the comprehension of signal transduction events preceding or concomitant to the angiogenic process required dramatic advancements in the methodology employed. We will describe the main techniques employed to evaluate the connection of endothelial cell signal transduction to angiogenesis, focusing on their strength and limitations.

Original languageEnglish
Title of host publicationAngiogenesis Assays: A Critical Appraisal of Current Techniques
PublisherJohn Wiley & Sons, Ltd
Pages139-166
Number of pages28
ISBN (Print)0470016000, 9780470016008
DOIs
Publication statusPublished - Sep 27 2007

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Keywords

  • Antisense and siRNA
  • Chorioallantoic membrane (CAM) in vivo
  • Endothelial nitric oxide synthase (eNOS)
  • Fluorescence resonance energy transfer (FRET)
  • Membrane and intracellular signalling events assays
  • Signalling protein complexes in membrane microdomains
  • Vascular endothelium growth factor (VEGF)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tomasi, V., Griffoni, C., Santi, S., Lenaz, P., Iorio, R. A., Strillacci, A., & Spisni, E. (2007). Assays for Membrane and Intracellular Signalling Events. In Angiogenesis Assays: A Critical Appraisal of Current Techniques (pp. 139-166). John Wiley & Sons, Ltd. https://doi.org/10.1002/9780470029350.ch8