TY - JOUR
T1 - Assembly and characterization of pandemic influenza A H1N1 genome in nasopharyngeal swabs using high-throughput pyrosequencing
AU - Bartolini, Barbara
AU - Chillemi, Giovanni
AU - Abbate, Isabella
AU - Bruselles, Alessandro
AU - Rozera, Gabriella
AU - Castrignanò, Tiziana
AU - Paoletti, Daniele
AU - Picardi, Ernesto
AU - Desideri, Alessandro
AU - Pesole, Graziano
AU - Capobianchi, Maria R.
PY - 2011
Y1 - 2011
N2 - De novo high-throughput pyrosequencing was used to detect and characterize 2009 pandemic influenza A (H1N1) virus directly in nasopharyngeal swabs in the context of the microbial community. Data were generated with a prior sequenceindependent amplification by 454 pyrosequencing on GS-FLX platform (Roche). Influenza A assembled reads allowed near full-length genome reconstruction with the simultaneous analysis of site-specific heterogeneity. The molecular approach applied proved to be a powerful tool to characterize the new pandemic H1N1 influenza virus in clinical samples. This approach could be of great value in identifying possibly new reassortants that may occur in the near future.
AB - De novo high-throughput pyrosequencing was used to detect and characterize 2009 pandemic influenza A (H1N1) virus directly in nasopharyngeal swabs in the context of the microbial community. Data were generated with a prior sequenceindependent amplification by 454 pyrosequencing on GS-FLX platform (Roche). Influenza A assembled reads allowed near full-length genome reconstruction with the simultaneous analysis of site-specific heterogeneity. The molecular approach applied proved to be a powerful tool to characterize the new pandemic H1N1 influenza virus in clinical samples. This approach could be of great value in identifying possibly new reassortants that may occur in the near future.
KW - High-throughput pyrosequencing
KW - Influenza
KW - Nasopharyngeal swabs
UR - http://www.scopus.com/inward/record.url?scp=82755174195&partnerID=8YFLogxK
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M3 - Article
C2 - 22143812
AN - SCOPUS:82755174195
VL - 34
SP - 391
EP - 397
JO - New Microbiologica
JF - New Microbiologica
SN - 1121-7138
IS - 4
ER -