TY - JOUR
T1 - Assessing free-radical-mediated DNA damage during cardiac surgery
T2 - 8-oxo-7,8-dihydro-2′-deoxyguanosine as a putative biomarker
AU - Turnu, Linda
AU - Di Minno, Alessandro
AU - Porro, Benedetta
AU - Squellerio, Isabella
AU - Bonomi, Alice
AU - Manega, Chiara Maria
AU - Werba, José Pablo
AU - Parolari, Alessandro
AU - Tremoli, Elena
AU - Cavalca, Viviana
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Coronary artery bypass grafting (CABG), one of the most common cardiac surgical procedures, is characterized by a burst of oxidative stress. 8-Oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), produced following DNA repairing, is used as an indicator of oxidative DNA damage in humans. The effect of CABG on oxidative-induced DNA damage, evaluated through the measurement of urinary 8-oxodG by a developed and validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method in 52 coronary artery disease (CAD) patients, was assessed before (T0), five days (T1), and six months (T2) after CABG procedure. These results were compared with those obtained in 40 subjects with cardiovascular risk factors and without overt cardiovascular disease (CTR). Baseline (T0) 8-oxodG was higher in CAD than in CTR (p=0.035). A significant burst was detected at T1 (p=0.019), while at T2, 8-oxodG levels were significantly lower than those measured at T0 (p<0.0001) and comparable to those found in CTR (p=0.73). A similar trend was observed for urinary 8-iso-prostaglandin F2 (8-isoPGF2), a reliable marker of oxidative stress. In the whole population baseline, 8-oxodG significantly correlated with 8-isoPGF2 levels (r=0.323, p=0.002). These data argue for CABG procedure in CAD patients as inducing a short-term increase in oxidative DNA damage, as revealed by 8-oxodG concentrations, and a long-term return of such metabolite toward physiological levels.
AB - Coronary artery bypass grafting (CABG), one of the most common cardiac surgical procedures, is characterized by a burst of oxidative stress. 8-Oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), produced following DNA repairing, is used as an indicator of oxidative DNA damage in humans. The effect of CABG on oxidative-induced DNA damage, evaluated through the measurement of urinary 8-oxodG by a developed and validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method in 52 coronary artery disease (CAD) patients, was assessed before (T0), five days (T1), and six months (T2) after CABG procedure. These results were compared with those obtained in 40 subjects with cardiovascular risk factors and without overt cardiovascular disease (CTR). Baseline (T0) 8-oxodG was higher in CAD than in CTR (p=0.035). A significant burst was detected at T1 (p=0.019), while at T2, 8-oxodG levels were significantly lower than those measured at T0 (p<0.0001) and comparable to those found in CTR (p=0.73). A similar trend was observed for urinary 8-iso-prostaglandin F2 (8-isoPGF2), a reliable marker of oxidative stress. In the whole population baseline, 8-oxodG significantly correlated with 8-isoPGF2 levels (r=0.323, p=0.002). These data argue for CABG procedure in CAD patients as inducing a short-term increase in oxidative DNA damage, as revealed by 8-oxodG concentrations, and a long-term return of such metabolite toward physiological levels.
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U2 - 10.1155/2017/9715898
DO - 10.1155/2017/9715898
M3 - Article
AN - SCOPUS:85021638269
VL - 2017
JO - Oxidative Medicine and Cellular Longevity
JF - Oxidative Medicine and Cellular Longevity
SN - 1942-0900
M1 - 9715898
ER -