Assessing Free-Radical-Mediated DNA Damage during Cardiac Surgery

8-Oxo-7,8-dihydro-2'-deoxyguanosine as a Putative Biomarker

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Abstract

Coronary artery bypass grafting (CABG), one of the most common cardiac surgical procedures, is characterized by a burst of oxidative stress. 8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), produced following DNA repairing, is used as an indicator of oxidative DNA damage in humans. The effect of CABG on oxidative-induced DNA damage, evaluated through the measurement of urinary 8-oxodG by a developed and validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method in 52 coronary artery disease (CAD) patients, was assessed before (T0), five days (T1), and six months (T2) after CABG procedure. These results were compared with those obtained in 40 subjects with cardiovascular risk factors and without overt cardiovascular disease (CTR). Baseline (T0) 8-oxodG was higher in CAD than in CTR (p= 0.035). A significant burst was detected at T1 (p= 0.019), while at T2, 8-oxodG levels were significantly lower than those measured at T0 (p< 0.0001) and comparable to those found in CTR (p= 0.73). A similar trend was observed for urinary 8-iso-prostaglandin F2α(8-isoPGF2α), a reliable marker of oxidative stress. In the whole population baseline, 8-oxodG significantly correlated with 8-isoPGF2αlevels (r= 0.323,p= 0.002). These data argue for CABG procedure in CAD patients as inducing a short-term increase in oxidative DNA damage, as revealed by 8-oxodG concentrations, and a long-term return of such metabolite toward physiological levels.

Original languageEnglish
Pages (from-to)9715898
JournalOxidative Medicine and Cellular Longevity
Volume2017
DOIs
Publication statusPublished - 2017

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Biomarkers
Surgery
Thoracic Surgery
DNA Damage
Free Radicals
Coronary Artery Bypass
DNA
Coronary Artery Disease
Oxidative stress
Oxidative Stress
Cardiac Surgical Procedures
Dinoprost
Liquid chromatography
Metabolites
Tandem Mass Spectrometry
Liquid Chromatography
Mass spectrometry
8-oxo-7-hydrodeoxyguanosine
Cardiovascular Diseases
Population

Cite this

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title = "Assessing Free-Radical-Mediated DNA Damage during Cardiac Surgery: 8-Oxo-7,8-dihydro-2'-deoxyguanosine as a Putative Biomarker",
abstract = "Coronary artery bypass grafting (CABG), one of the most common cardiac surgical procedures, is characterized by a burst of oxidative stress. 8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), produced following DNA repairing, is used as an indicator of oxidative DNA damage in humans. The effect of CABG on oxidative-induced DNA damage, evaluated through the measurement of urinary 8-oxodG by a developed and validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method in 52 coronary artery disease (CAD) patients, was assessed before (T0), five days (T1), and six months (T2) after CABG procedure. These results were compared with those obtained in 40 subjects with cardiovascular risk factors and without overt cardiovascular disease (CTR). Baseline (T0) 8-oxodG was higher in CAD than in CTR (p= 0.035). A significant burst was detected at T1 (p= 0.019), while at T2, 8-oxodG levels were significantly lower than those measured at T0 (p< 0.0001) and comparable to those found in CTR (p= 0.73). A similar trend was observed for urinary 8-iso-prostaglandin F2α(8-isoPGF2α), a reliable marker of oxidative stress. In the whole population baseline, 8-oxodG significantly correlated with 8-isoPGF2αlevels (r= 0.323,p= 0.002). These data argue for CABG procedure in CAD patients as inducing a short-term increase in oxidative DNA damage, as revealed by 8-oxodG concentrations, and a long-term return of such metabolite toward physiological levels.",
author = "Linda Turnu and {Di Minno}, Alessandro and Benedetta Porro and Isabella Squellerio and Alice Bonomi and Manega, {Chiara Maria} and Werba, {Jos{\'e} Pablo} and Alessandro Parolari and Elena Tremoli and Viviana Cavalca",
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T2 - 8-Oxo-7,8-dihydro-2'-deoxyguanosine as a Putative Biomarker

AU - Turnu, Linda

AU - Di Minno, Alessandro

AU - Porro, Benedetta

AU - Squellerio, Isabella

AU - Bonomi, Alice

AU - Manega, Chiara Maria

AU - Werba, José Pablo

AU - Parolari, Alessandro

AU - Tremoli, Elena

AU - Cavalca, Viviana

PY - 2017

Y1 - 2017

N2 - Coronary artery bypass grafting (CABG), one of the most common cardiac surgical procedures, is characterized by a burst of oxidative stress. 8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), produced following DNA repairing, is used as an indicator of oxidative DNA damage in humans. The effect of CABG on oxidative-induced DNA damage, evaluated through the measurement of urinary 8-oxodG by a developed and validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method in 52 coronary artery disease (CAD) patients, was assessed before (T0), five days (T1), and six months (T2) after CABG procedure. These results were compared with those obtained in 40 subjects with cardiovascular risk factors and without overt cardiovascular disease (CTR). Baseline (T0) 8-oxodG was higher in CAD than in CTR (p= 0.035). A significant burst was detected at T1 (p= 0.019), while at T2, 8-oxodG levels were significantly lower than those measured at T0 (p< 0.0001) and comparable to those found in CTR (p= 0.73). A similar trend was observed for urinary 8-iso-prostaglandin F2α(8-isoPGF2α), a reliable marker of oxidative stress. In the whole population baseline, 8-oxodG significantly correlated with 8-isoPGF2αlevels (r= 0.323,p= 0.002). These data argue for CABG procedure in CAD patients as inducing a short-term increase in oxidative DNA damage, as revealed by 8-oxodG concentrations, and a long-term return of such metabolite toward physiological levels.

AB - Coronary artery bypass grafting (CABG), one of the most common cardiac surgical procedures, is characterized by a burst of oxidative stress. 8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), produced following DNA repairing, is used as an indicator of oxidative DNA damage in humans. The effect of CABG on oxidative-induced DNA damage, evaluated through the measurement of urinary 8-oxodG by a developed and validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method in 52 coronary artery disease (CAD) patients, was assessed before (T0), five days (T1), and six months (T2) after CABG procedure. These results were compared with those obtained in 40 subjects with cardiovascular risk factors and without overt cardiovascular disease (CTR). Baseline (T0) 8-oxodG was higher in CAD than in CTR (p= 0.035). A significant burst was detected at T1 (p= 0.019), while at T2, 8-oxodG levels were significantly lower than those measured at T0 (p< 0.0001) and comparable to those found in CTR (p= 0.73). A similar trend was observed for urinary 8-iso-prostaglandin F2α(8-isoPGF2α), a reliable marker of oxidative stress. In the whole population baseline, 8-oxodG significantly correlated with 8-isoPGF2αlevels (r= 0.323,p= 0.002). These data argue for CABG procedure in CAD patients as inducing a short-term increase in oxidative DNA damage, as revealed by 8-oxodG concentrations, and a long-term return of such metabolite toward physiological levels.

U2 - 10.1155/2017/9715898

DO - 10.1155/2017/9715898

M3 - Article

VL - 2017

SP - 9715898

JO - Oxidative Medicine and Cellular Longevity

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SN - 1942-0900

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