Assessing Pseudomonas aeruginosa virulence and the host response using Murine models of acute and chronic lung infection

Irena Kukavica-Ibrulj, Marcella Facchini, Cristina Cigana, Roger C. Levesque, Alessandra Bragonzi

Research output: Contribution to journalArticlepeer-review

Abstract

Murine models of acute and chronic lung infection have been used in studying Pseudomonas aeruginosa for assessing in vivo behavior and for monitoring of the host response. These models provide an important resource for studies of the initiation and maintenance of bacterial infection, identify bacterial genes essential for in vivo maintenance and for the development and testing of new therapies. The rat has been used extensively as a model of chronic lung infection, whereas the mouse has been a model of acute and chronic infection. Intratracheal administration of planktonic bacterial cells in the mouse provides a model of acute pneumonia. Bacteria enmeshed in agar beads can be used in the rat and mouse to reproduce the lung pathology of cystic fibrosis patients with advanced chronic pulmonary disease. Here, we describe the methods to assess virulence of P. aeruginosa using prototype and clinical strains in the Sprague-Dawley rat and the C57BL/6NCrlBR mouse by monitoring several measurable read-outs including weight loss, mortality, in vivo growth curves, the competitive index of infectivity, and the inflammatory response.

Original languageEnglish
Pages (from-to)757-771
Number of pages15
JournalMethods in molecular biology (Clifton, N.J.)
Volume1149
DOIs
Publication statusPublished - 2014

Keywords

  • Acute
  • Chronic
  • Cystic fibrosis
  • Lung infection
  • Murine models
  • Pneumonia
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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