TY - JOUR
T1 - Assessing Pseudomonas aeruginosa virulence and the host response using Murine models of acute and chronic lung infection
AU - Kukavica-Ibrulj, Irena
AU - Facchini, Marcella
AU - Cigana, Cristina
AU - Levesque, Roger C.
AU - Bragonzi, Alessandra
PY - 2014
Y1 - 2014
N2 - Murine models of acute and chronic lung infection have been used in studying Pseudomonas aeruginosa for assessing in vivo behavior and for monitoring of the host response. These models provide an important resource for studies of the initiation and maintenance of bacterial infection, identify bacterial genes essential for in vivo maintenance and for the development and testing of new therapies. The rat has been used extensively as a model of chronic lung infection, whereas the mouse has been a model of acute and chronic infection. Intratracheal administration of planktonic bacterial cells in the mouse provides a model of acute pneumonia. Bacteria enmeshed in agar beads can be used in the rat and mouse to reproduce the lung pathology of cystic fibrosis patients with advanced chronic pulmonary disease. Here, we describe the methods to assess virulence of P. aeruginosa using prototype and clinical strains in the Sprague-Dawley rat and the C57BL/6NCrlBR mouse by monitoring several measurable read-outs including weight loss, mortality, in vivo growth curves, the competitive index of infectivity, and the inflammatory response.
AB - Murine models of acute and chronic lung infection have been used in studying Pseudomonas aeruginosa for assessing in vivo behavior and for monitoring of the host response. These models provide an important resource for studies of the initiation and maintenance of bacterial infection, identify bacterial genes essential for in vivo maintenance and for the development and testing of new therapies. The rat has been used extensively as a model of chronic lung infection, whereas the mouse has been a model of acute and chronic infection. Intratracheal administration of planktonic bacterial cells in the mouse provides a model of acute pneumonia. Bacteria enmeshed in agar beads can be used in the rat and mouse to reproduce the lung pathology of cystic fibrosis patients with advanced chronic pulmonary disease. Here, we describe the methods to assess virulence of P. aeruginosa using prototype and clinical strains in the Sprague-Dawley rat and the C57BL/6NCrlBR mouse by monitoring several measurable read-outs including weight loss, mortality, in vivo growth curves, the competitive index of infectivity, and the inflammatory response.
KW - Acute
KW - Chronic
KW - Cystic fibrosis
KW - Lung infection
KW - Murine models
KW - Pneumonia
KW - Pseudomonas aeruginosa
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U2 - 10.1007/978-1-4939-0473-0_58
DO - 10.1007/978-1-4939-0473-0_58
M3 - Article
C2 - 24818948
AN - SCOPUS:84925883898
VL - 1149
SP - 757
EP - 771
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -