Assessing the anti-tumour properties of Iraqi propolis in vitro and in vivo

Ghassan M. Sulaiman, Ali H. Ad'hiah, Khulood W. Al-Sammarrae, Renzo Bagnati, Roberta Frapolli, Ezia Bello, Sarah Uboldi, Michela Romano, Nicolò Panini, Eugenio Scanziani, Marzia Pezzolato, Eugenio Erba, Maurizio D'Incalci

Research output: Contribution to journalArticle

Abstract

The study was designed to evaluate anti-tumour properties of Iraqi propolis collected from Mosul region (M) on HL-60 and HCT-116 cell lines and on HCT-116 in vivo. M induced an inhibitory effect against the proliferation of HL-60 and colony potential of HCT-116 cells. The apoptosis in HL-60 cells was associated with down-regulation of Bcl-2 and activation of Bax, while in HCT-116 cells, necrotic features were observed; size of cells was dramatically increased by swelling of cytoplasm and loss of membrane integrity, cell rupture and release of cellular contents. Analysis of BrdU/DNA cell cycle in both cell lines showed that M induced cell cycle perturbations in both BrdU positive and BrdU negative cells. The exposure of HL-60 to M caused γ-H2AX in a dose dependent manner and was associated with induction of apoptosis. The experiments in HCT-116 tumor-bearing mice showed that oral administration of propolis at doses that caused no detectable toxicity was associated with a decrease in mitotic cells and an increase in endoreduplications, increased p53 and decreased Ki-67 expression of cells in tumor sections. This study provides the rationale to investigate the potential beneficial effect of propolis in the diet of patients receiving anti-cancer therapies.

Original languageEnglish
Pages (from-to)1632-1641
Number of pages10
JournalFood and Chemical Toxicology
Volume50
Issue number5
DOIs
Publication statusPublished - May 2012

Fingerprint

Propolis
propolis
HCT116 Cells
Tumors
Bromodeoxyuridine
Cells
neoplasms
Neoplasms
Cell Cycle
Endoreduplication
cells
Apoptosis
Cell Line
Bearings (structural)
HL-60 Cells
Cell Size
cell cycle
apoptosis
Oral Administration
Rupture

Keywords

  • BrdU/DNA cell cycle
  • Cytotoxicity
  • Iraqi propolis

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Assessing the anti-tumour properties of Iraqi propolis in vitro and in vivo. / Sulaiman, Ghassan M.; Ad'hiah, Ali H.; Al-Sammarrae, Khulood W.; Bagnati, Renzo; Frapolli, Roberta; Bello, Ezia; Uboldi, Sarah; Romano, Michela; Panini, Nicolò; Scanziani, Eugenio; Pezzolato, Marzia; Erba, Eugenio; D'Incalci, Maurizio.

In: Food and Chemical Toxicology, Vol. 50, No. 5, 05.2012, p. 1632-1641.

Research output: Contribution to journalArticle

Sulaiman, GM, Ad'hiah, AH, Al-Sammarrae, KW, Bagnati, R, Frapolli, R, Bello, E, Uboldi, S, Romano, M, Panini, N, Scanziani, E, Pezzolato, M, Erba, E & D'Incalci, M 2012, 'Assessing the anti-tumour properties of Iraqi propolis in vitro and in vivo', Food and Chemical Toxicology, vol. 50, no. 5, pp. 1632-1641. https://doi.org/10.1016/j.fct.2012.01.022
Sulaiman, Ghassan M. ; Ad'hiah, Ali H. ; Al-Sammarrae, Khulood W. ; Bagnati, Renzo ; Frapolli, Roberta ; Bello, Ezia ; Uboldi, Sarah ; Romano, Michela ; Panini, Nicolò ; Scanziani, Eugenio ; Pezzolato, Marzia ; Erba, Eugenio ; D'Incalci, Maurizio. / Assessing the anti-tumour properties of Iraqi propolis in vitro and in vivo. In: Food and Chemical Toxicology. 2012 ; Vol. 50, No. 5. pp. 1632-1641.
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