Assessing Tumor-infiltrating Lymphocytes in Solid Tumors: A Practical Review for Pathologists and Proposal for a Standardized Method From the International Immunooncology Biomarkers Working Group: Part 1: Assessing the Host Immune Response, TILs in Invasive Breast Carcinoma and Ductal Carcinoma In Situ, Metastatic Tumor Deposits and Areas for Further Research

Shona Hendry, Roberto Salgado, Thomas Gevaert, Prudence A Russell, Tom John, Bibhusal Thapa, Michael Christie, Koen van de Vijver, M V Estrada, Paula I Gonzalez-Ericsson, Melinda Sanders, Benjamin Solomon, Cinzia Solinas, Gert G G M Van den Eynden, Yves Allory, Matthias Preusser, Johannes Hainfellner, Giancarlo Pruneri, Andrea Vingiani, Sandra DemariaFraser Symmans, Paolo Nuciforo, Laura Comerma, E A Thompson, Sunil Lakhani, Seong-Rim Kim, Stuart Schnitt, Cecile Colpaert, Christos Sotiriou, Stefan J Scherer, Michail Ignatiadis, Sunil Badve, Robert H Pierce, Giuseppe Viale, Nicolas Sirtaine, Frederique Penault-Llorca, Tomohagu Sugie, Susan Fineberg, Soonmyung Paik, Ashok Srinivasan, Andrea Richardson, Yihong Wang, Ewa Chmielik, Jane Brock, Douglas B Johnson, Justin Balko, Stephan Wienert, Carmen Criscitiello, Giuseppe Curigliano, Maria V Dieci

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Abstract

Assessment of tumor-infiltrating lymphocytes (TILs) in histopathologic specimens can provide important prognostic information in diverse solid tumor types, and may also be of value in predicting response to treatments. However, implementation as a routine clinical biomarker has not yet been achieved. As successful use of immune checkpoint inhibitors and other forms of immunotherapy become a clinical reality, the need for widely applicable, accessible, and reliable immunooncology biomarkers is clear. In part 1 of this review we briefly discuss the host immune response to tumors and different approaches to TIL assessment. We propose a standardized methodology to assess TILs in solid tumors on hematoxylin and eosin sections, in both primary and metastatic settings, based on the International Immuno-Oncology Biomarker Working Group guidelines for TIL assessment in invasive breast carcinoma. A review of the literature regarding the value of TIL assessment in different solid tumor types follows in part 2. The method we propose is reproducible, affordable, easily applied, and has demonstrated prognostic and predictive significance in invasive breast carcinoma. This standardized methodology may be used as a reference against which other methods are compared, and should be evaluated for clinical validity and utility. Standardization of TIL assessment will help to improve consistency and reproducibility in this field, enrich both the quality and quantity of comparable evidence, and help to thoroughly evaluate the utility of TILs assessment in this era of immunotherapy.

Original languageEnglish
Pages (from-to)235-251
Number of pages17
JournalAdvances in Anatomic Pathology
Volume24
Issue number5
DOIs
Publication statusPublished - Sep 2017

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Tumor-Infiltrating Lymphocytes
Carcinoma, Intraductal, Noninfiltrating
Biomarkers
Breast Neoplasms
Research
Neoplasms
Immunotherapy
Hematoxylin
Eosine Yellowish-(YS)
Pathologists
Guidelines

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Assessing Tumor-infiltrating Lymphocytes in Solid Tumors : A Practical Review for Pathologists and Proposal for a Standardized Method From the International Immunooncology Biomarkers Working Group: Part 1: Assessing the Host Immune Response, TILs in Invasive Breast Carcinoma and Ductal Carcinoma In Situ, Metastatic Tumor Deposits and Areas for Further Research. / Hendry, Shona; Salgado, Roberto; Gevaert, Thomas; Russell, Prudence A; John, Tom; Thapa, Bibhusal; Christie, Michael; van de Vijver, Koen; Estrada, M V; Gonzalez-Ericsson, Paula I; Sanders, Melinda; Solomon, Benjamin; Solinas, Cinzia; Van den Eynden, Gert G G M; Allory, Yves; Preusser, Matthias; Hainfellner, Johannes; Pruneri, Giancarlo; Vingiani, Andrea; Demaria, Sandra; Symmans, Fraser; Nuciforo, Paolo; Comerma, Laura; Thompson, E A; Lakhani, Sunil; Kim, Seong-Rim; Schnitt, Stuart; Colpaert, Cecile; Sotiriou, Christos; Scherer, Stefan J; Ignatiadis, Michail; Badve, Sunil; Pierce, Robert H; Viale, Giuseppe; Sirtaine, Nicolas; Penault-Llorca, Frederique; Sugie, Tomohagu; Fineberg, Susan; Paik, Soonmyung; Srinivasan, Ashok; Richardson, Andrea; Wang, Yihong; Chmielik, Ewa; Brock, Jane; Johnson, Douglas B; Balko, Justin; Wienert, Stephan; Criscitiello, Carmen; Curigliano, Giuseppe; Dieci, Maria V.

In: Advances in Anatomic Pathology, Vol. 24, No. 5, 09.2017, p. 235-251.

Research output: Contribution to journalArticle

Hendry, S, Salgado, R, Gevaert, T, Russell, PA, John, T, Thapa, B, Christie, M, van de Vijver, K, Estrada, MV, Gonzalez-Ericsson, PI, Sanders, M, Solomon, B, Solinas, C, Van den Eynden, GGGM, Allory, Y, Preusser, M, Hainfellner, J, Pruneri, G, Vingiani, A, Demaria, S, Symmans, F, Nuciforo, P, Comerma, L, Thompson, EA, Lakhani, S, Kim, S-R, Schnitt, S, Colpaert, C, Sotiriou, C, Scherer, SJ, Ignatiadis, M, Badve, S, Pierce, RH, Viale, G, Sirtaine, N, Penault-Llorca, F, Sugie, T, Fineberg, S, Paik, S, Srinivasan, A, Richardson, A, Wang, Y, Chmielik, E, Brock, J, Johnson, DB, Balko, J, Wienert, S, Criscitiello, C, Curigliano, G & Dieci, MV 2017, 'Assessing Tumor-infiltrating Lymphocytes in Solid Tumors: A Practical Review for Pathologists and Proposal for a Standardized Method From the International Immunooncology Biomarkers Working Group: Part 1: Assessing the Host Immune Response, TILs in Invasive Breast Carcinoma and Ductal Carcinoma In Situ, Metastatic Tumor Deposits and Areas for Further Research', Advances in Anatomic Pathology, vol. 24, no. 5, pp. 235-251. https://doi.org/10.1097/PAP.0000000000000162
Hendry, Shona ; Salgado, Roberto ; Gevaert, Thomas ; Russell, Prudence A ; John, Tom ; Thapa, Bibhusal ; Christie, Michael ; van de Vijver, Koen ; Estrada, M V ; Gonzalez-Ericsson, Paula I ; Sanders, Melinda ; Solomon, Benjamin ; Solinas, Cinzia ; Van den Eynden, Gert G G M ; Allory, Yves ; Preusser, Matthias ; Hainfellner, Johannes ; Pruneri, Giancarlo ; Vingiani, Andrea ; Demaria, Sandra ; Symmans, Fraser ; Nuciforo, Paolo ; Comerma, Laura ; Thompson, E A ; Lakhani, Sunil ; Kim, Seong-Rim ; Schnitt, Stuart ; Colpaert, Cecile ; Sotiriou, Christos ; Scherer, Stefan J ; Ignatiadis, Michail ; Badve, Sunil ; Pierce, Robert H ; Viale, Giuseppe ; Sirtaine, Nicolas ; Penault-Llorca, Frederique ; Sugie, Tomohagu ; Fineberg, Susan ; Paik, Soonmyung ; Srinivasan, Ashok ; Richardson, Andrea ; Wang, Yihong ; Chmielik, Ewa ; Brock, Jane ; Johnson, Douglas B ; Balko, Justin ; Wienert, Stephan ; Criscitiello, Carmen ; Curigliano, Giuseppe ; Dieci, Maria V. / Assessing Tumor-infiltrating Lymphocytes in Solid Tumors : A Practical Review for Pathologists and Proposal for a Standardized Method From the International Immunooncology Biomarkers Working Group: Part 1: Assessing the Host Immune Response, TILs in Invasive Breast Carcinoma and Ductal Carcinoma In Situ, Metastatic Tumor Deposits and Areas for Further Research. In: Advances in Anatomic Pathology. 2017 ; Vol. 24, No. 5. pp. 235-251.
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