Assessment of blood-brain barrier permeability by intravenous infusion of FITC-labeled albumin in a mouse model of neurodegenerative disease

Alba Di Pardo, Salvatore Castaldo, Luca Capocci, Enrico Amico, Maglione Vittorio

Research output: Contribution to journalArticle

Abstract

Disruption of blood-brain barrier (BBB) integrity is a common feature for different neurological and neurodegenerative diseases. Although the interplay between perturbed BBB homeostasis and the pathogenesis of brain disorders needs further investigation, the development and validation of a reliable procedure to accurately detect BBB alterations may be crucial and represent a useful tool for potentially predicting disease progression and developing targeted therapeutic strategies. Here, we present an easy and efficient procedure for evaluating BBB leakage in a neurodegenerative condition like that occurring in a preclinical mouse model of Huntington disease, in which defects in the permeability of BBB are clearly detectable precociously in the disease. Specifically, the high molecular weight fluorescein isothiocyanate labelled (FITC)-albumin, which is able to cross the BBB only when the latter is impaired, is acutely infused into a mouse jugular vein and its distribution in the vascular or parenchymal districts is then determined by fluorescence microscopy. Accumulation of green fluorescent-albumin in the brain parenchyma functions as an index of aberrant BBB permeability and, when quantitated by using Image J processing software, is reported as Green Fluorescence Intensity.

Original languageEnglish
Article numbere56389
JournalJournal of Visualized Experiments
Volume2017
Issue number129
DOIs
Publication statusPublished - Nov 8 2017

Keywords

  • BBB permeability
  • Blood-Brain Barrier (BBB)
  • FITC-albumin
  • Intravenous injection
  • Issue 129
  • Mouse model
  • Neurodegeneration
  • Neuroscience

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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