TY - JOUR
T1 - Assessment of bone marrow involvement in non-Hodgkin's lymphomas
T2 - Comparison between histology and flow cytometry
AU - Merli, Michele
AU - Arcaini, Luca
AU - Boveri, Emanuela
AU - Rattotti, Sara
AU - Picone, Cristina
AU - Passamonti, Francesco
AU - Tenore, Annamaria
AU - Sozzani, Laura
AU - Lucioni, Marco
AU - Varettoni, Marzia
AU - Rizzi, Silvia
AU - Morello, Lucia
AU - Ferretti, Virginia
AU - Pascutto, Cristiana
AU - Paulli, Marco
AU - Lazzarino, Mario
PY - 2010/11
Y1 - 2010/11
N2 - Bone marrow (BM) examination is essential in the staging of non-Hodgkin's lymphoma (NHL) patients. Few studies have compared BM histologic findings with results of flow cytometric (FC) analysis. We analyzed the incidence and patterns of histologic BM involvement in a series of 753 patients with NHL. For 498 patients, a concurrent FC analysis on BM was available. Histologic involvement was detected at diagnosis in 311/753 (41%) patients. By FC, BM involvement was clearly detected in 150/498 (30%). After excluding 12 cases with equivocal histology, concordance between the two methods was detected in 411 (85%) cases (27% BMB+/FC+; 58% BMB-/FC-), while discordance was present in 75 (15%) (P <0.001): 58 cases (12%) were BMB+/FC- and 17 (3%) were BMB-/FC+. Discordance was more frequent in FL and in lymphoplasmacytic lymphoma (LPL). These data demonstrate that the two methods are comparable in qualitative assessment of BM involvement in NHL, with the exception of FL and LPL. In FL, diffuse large B-cell lymphoma (DLBCL) and LPL, FC underestimates the extent of infiltrate with respect to histology.
AB - Bone marrow (BM) examination is essential in the staging of non-Hodgkin's lymphoma (NHL) patients. Few studies have compared BM histologic findings with results of flow cytometric (FC) analysis. We analyzed the incidence and patterns of histologic BM involvement in a series of 753 patients with NHL. For 498 patients, a concurrent FC analysis on BM was available. Histologic involvement was detected at diagnosis in 311/753 (41%) patients. By FC, BM involvement was clearly detected in 150/498 (30%). After excluding 12 cases with equivocal histology, concordance between the two methods was detected in 411 (85%) cases (27% BMB+/FC+; 58% BMB-/FC-), while discordance was present in 75 (15%) (P <0.001): 58 cases (12%) were BMB+/FC- and 17 (3%) were BMB-/FC+. Discordance was more frequent in FL and in lymphoplasmacytic lymphoma (LPL). These data demonstrate that the two methods are comparable in qualitative assessment of BM involvement in NHL, with the exception of FL and LPL. In FL, diffuse large B-cell lymphoma (DLBCL) and LPL, FC underestimates the extent of infiltrate with respect to histology.
KW - Bone marrow histology
KW - Flow cytometry
KW - Non-Hodgkin's lymphoma
KW - Staging
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U2 - 10.1111/j.1600-0609.2010.01503.x
DO - 10.1111/j.1600-0609.2010.01503.x
M3 - Article
C2 - 20662897
AN - SCOPUS:77958550221
VL - 85
SP - 405
EP - 415
JO - European Journal of Haematology
JF - European Journal of Haematology
SN - 0902-4441
IS - 5
ER -