Assessment of dual-energy x-ray absorptiometry for use in evaluating the effects of dietary and environmental management on hermann's tortoises (Testudo hermanni)

Matteo Gramanzini, Nicola Di Girolamo, Sara Gargiulo, Adelaide Greco, Natascia Cocchia, Mauro Delogu, Isabella Rosapane, Raffaele Liuzzi, Paolo Selleri, Arturo Brunetti

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective-To assess dual-energy x-ray absorptiometry (DXA) for evaluating effects of diet and environment on bone mineral density in Hermann's tortoises (Testudo hermanni). Animals-26 Hermann's tortoises within 1 month after hatching. Procedures-Group 1 was housed in an artificial setting and fed naturally growing vegetation. Group 2 was housed in an artificial setting and fed vegetables grown for human consumption. Group 3 was maintained in an outside enclosure and fed naturally growing vegetation. After 10 months, pyramidal growth, body weight, and adverse conditions were assessed. Bone mineral density (BMD) of the axial and appendicular skeleton, shell, vertebral column, and pelvis was measured via DXA. Results-Group 2 had the highest mean ± SD body weight (65.42 ± 30.85 g), followed by group 1 (51.08 ± 22.92 g) and group 3 (35.74 ± 7.13 g). Mean BMD of the shell varied significantly among groups (group 1, 0.05 ± 0.03 g/cm2•m; group 2, 0.09 ± 0.15 g/cm2•m; and group 3, undetectable). The BMD of the axial and appendicular skeleton, vertebral column, and pelvis did not differ significantly among groups. Pyramidal growth was highest in group 1 and not evident in group 3. Conclusions and Clinical Relevance-Tortoises raised in artificial conditions did not have deficits in BMD, compared with results for outdoor-housed hibernating tortoises. Supplemental calcium was apparently not necessary when an adequate photothermal habitat and plant-based diet were provided. Higher BMD of captive-raised tortoises was morphologically associated with a higher incidence of pyramidal growth in captive-raised groups.

Original languageEnglish
Pages (from-to)918-924
Number of pages7
JournalAmerican Journal of Veterinary Research
Volume74
Issue number6
DOIs
Publication statusPublished - Jun 2013

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Turtles
tortoises
environmental management
bone density
Bone Density
X-radiation
X-Rays
energy
pelvis
spine (bones)
Pelvis
Skeleton
skeleton
Spine
Growth
Body Weight
plant-based diet
Diet
vegetation
body weight

ASJC Scopus subject areas

  • veterinary(all)

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Assessment of dual-energy x-ray absorptiometry for use in evaluating the effects of dietary and environmental management on hermann's tortoises (Testudo hermanni). / Gramanzini, Matteo; Di Girolamo, Nicola; Gargiulo, Sara; Greco, Adelaide; Cocchia, Natascia; Delogu, Mauro; Rosapane, Isabella; Liuzzi, Raffaele; Selleri, Paolo; Brunetti, Arturo.

In: American Journal of Veterinary Research, Vol. 74, No. 6, 06.2013, p. 918-924.

Research output: Contribution to journalArticle

Gramanzini, M, Di Girolamo, N, Gargiulo, S, Greco, A, Cocchia, N, Delogu, M, Rosapane, I, Liuzzi, R, Selleri, P & Brunetti, A 2013, 'Assessment of dual-energy x-ray absorptiometry for use in evaluating the effects of dietary and environmental management on hermann's tortoises (Testudo hermanni)', American Journal of Veterinary Research, vol. 74, no. 6, pp. 918-924. https://doi.org/10.2460/ajvr.74.6.918
Gramanzini, Matteo ; Di Girolamo, Nicola ; Gargiulo, Sara ; Greco, Adelaide ; Cocchia, Natascia ; Delogu, Mauro ; Rosapane, Isabella ; Liuzzi, Raffaele ; Selleri, Paolo ; Brunetti, Arturo. / Assessment of dual-energy x-ray absorptiometry for use in evaluating the effects of dietary and environmental management on hermann's tortoises (Testudo hermanni). In: American Journal of Veterinary Research. 2013 ; Vol. 74, No. 6. pp. 918-924.
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abstract = "Objective-To assess dual-energy x-ray absorptiometry (DXA) for evaluating effects of diet and environment on bone mineral density in Hermann's tortoises (Testudo hermanni). Animals-26 Hermann's tortoises within 1 month after hatching. Procedures-Group 1 was housed in an artificial setting and fed naturally growing vegetation. Group 2 was housed in an artificial setting and fed vegetables grown for human consumption. Group 3 was maintained in an outside enclosure and fed naturally growing vegetation. After 10 months, pyramidal growth, body weight, and adverse conditions were assessed. Bone mineral density (BMD) of the axial and appendicular skeleton, shell, vertebral column, and pelvis was measured via DXA. Results-Group 2 had the highest mean ± SD body weight (65.42 ± 30.85 g), followed by group 1 (51.08 ± 22.92 g) and group 3 (35.74 ± 7.13 g). Mean BMD of the shell varied significantly among groups (group 1, 0.05 ± 0.03 g/cm2•m; group 2, 0.09 ± 0.15 g/cm2•m; and group 3, undetectable). The BMD of the axial and appendicular skeleton, vertebral column, and pelvis did not differ significantly among groups. Pyramidal growth was highest in group 1 and not evident in group 3. Conclusions and Clinical Relevance-Tortoises raised in artificial conditions did not have deficits in BMD, compared with results for outdoor-housed hibernating tortoises. Supplemental calcium was apparently not necessary when an adequate photothermal habitat and plant-based diet were provided. Higher BMD of captive-raised tortoises was morphologically associated with a higher incidence of pyramidal growth in captive-raised groups.",
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AU - Gargiulo, Sara

AU - Greco, Adelaide

AU - Cocchia, Natascia

AU - Delogu, Mauro

AU - Rosapane, Isabella

AU - Liuzzi, Raffaele

AU - Selleri, Paolo

AU - Brunetti, Arturo

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