Abstract
Penconazole (PEN) is a fungicide used in agriculture. The aim of this work was to evaluate the exposure to PEN in vineyard workers focusing on urinary biomarkers. Twenty-two agricultural workers were involved in the study; they were investigated during PEN applications and re-entry work, performed for 1–4 consecutive working days, for a total of 42 mixing and applications and 12 re-entries. Potential and actual dermal exposure, including hand exposure, were measured using pads and hand washes. Urine samples were collected starting before the first application, continuing during the work shift, and ending 48 h after the last shift. The determination of PEN in dermal samples and PEN metabolites in urine was performed by liquid chromatography tandem mass spectrometry. Dermal potential body exposure and actual total exposure showed median levels ranging from 18 to 3356 µg and from 21 to 111 µg, respectively. Urinary monohydroxyl-derivative PEN-OH was the most abundant metabolite; its excretion rate peaked within 24 h after the work shift. In this period, median concentrations of PEN-OH and the carboxyl-derivative PEN-COOH ranged from 15.6 to 27.6 µg/L and from 2.5 to 10.2 µg/L, respectively. The concentration of PEN-OH during the work shift, in the 24 h after and in the 25–48 h after the work shift were correlated with actual body and total dermal exposure (Pearson's r from 0.279 to 0.562). Our results suggest that PEN-OH in the 24 h post-exposure urine is a promising candidate for biomonitoring PEN exposure in agricultural workers.
| Original language | English |
|---|---|
| Pages (from-to) | 54-61 |
| Number of pages | 8 |
| Journal | Environmental Research |
| Volume | 168 |
| DOIs | |
| Publication status | Published - Jan 1 2019 |
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Keywords
- Biomonitoring
- Dermal exposure
- Pesticides
- Urinary penconazole metabolites
- Vineyard workers
ASJC Scopus subject areas
- Biochemistry
- Environmental Science(all)
Cite this
Assessment of penconazole exposure in winegrowers using urinary biomarkers. / Mercadante, Rosa; Polledri, Elisa; Rubino, Federico Maria; Mandic-Rajcevic, Stefan; Vaiani, Andrea; Colosio, Claudio; Moretto, Angelo; Fustinoni, Silvia.
In: Environmental Research, Vol. 168, 01.01.2019, p. 54-61.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Assessment of penconazole exposure in winegrowers using urinary biomarkers
AU - Mercadante, Rosa
AU - Polledri, Elisa
AU - Rubino, Federico Maria
AU - Mandic-Rajcevic, Stefan
AU - Vaiani, Andrea
AU - Colosio, Claudio
AU - Moretto, Angelo
AU - Fustinoni, Silvia
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Penconazole (PEN) is a fungicide used in agriculture. The aim of this work was to evaluate the exposure to PEN in vineyard workers focusing on urinary biomarkers. Twenty-two agricultural workers were involved in the study; they were investigated during PEN applications and re-entry work, performed for 1–4 consecutive working days, for a total of 42 mixing and applications and 12 re-entries. Potential and actual dermal exposure, including hand exposure, were measured using pads and hand washes. Urine samples were collected starting before the first application, continuing during the work shift, and ending 48 h after the last shift. The determination of PEN in dermal samples and PEN metabolites in urine was performed by liquid chromatography tandem mass spectrometry. Dermal potential body exposure and actual total exposure showed median levels ranging from 18 to 3356 µg and from 21 to 111 µg, respectively. Urinary monohydroxyl-derivative PEN-OH was the most abundant metabolite; its excretion rate peaked within 24 h after the work shift. In this period, median concentrations of PEN-OH and the carboxyl-derivative PEN-COOH ranged from 15.6 to 27.6 µg/L and from 2.5 to 10.2 µg/L, respectively. The concentration of PEN-OH during the work shift, in the 24 h after and in the 25–48 h after the work shift were correlated with actual body and total dermal exposure (Pearson's r from 0.279 to 0.562). Our results suggest that PEN-OH in the 24 h post-exposure urine is a promising candidate for biomonitoring PEN exposure in agricultural workers.
AB - Penconazole (PEN) is a fungicide used in agriculture. The aim of this work was to evaluate the exposure to PEN in vineyard workers focusing on urinary biomarkers. Twenty-two agricultural workers were involved in the study; they were investigated during PEN applications and re-entry work, performed for 1–4 consecutive working days, for a total of 42 mixing and applications and 12 re-entries. Potential and actual dermal exposure, including hand exposure, were measured using pads and hand washes. Urine samples were collected starting before the first application, continuing during the work shift, and ending 48 h after the last shift. The determination of PEN in dermal samples and PEN metabolites in urine was performed by liquid chromatography tandem mass spectrometry. Dermal potential body exposure and actual total exposure showed median levels ranging from 18 to 3356 µg and from 21 to 111 µg, respectively. Urinary monohydroxyl-derivative PEN-OH was the most abundant metabolite; its excretion rate peaked within 24 h after the work shift. In this period, median concentrations of PEN-OH and the carboxyl-derivative PEN-COOH ranged from 15.6 to 27.6 µg/L and from 2.5 to 10.2 µg/L, respectively. The concentration of PEN-OH during the work shift, in the 24 h after and in the 25–48 h after the work shift were correlated with actual body and total dermal exposure (Pearson's r from 0.279 to 0.562). Our results suggest that PEN-OH in the 24 h post-exposure urine is a promising candidate for biomonitoring PEN exposure in agricultural workers.
KW - Biomonitoring
KW - Dermal exposure
KW - Pesticides
KW - Urinary penconazole metabolites
KW - Vineyard workers
UR - http://www.scopus.com/inward/record.url?scp=85053940643&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85053940643&partnerID=8YFLogxK
U2 - 10.1016/j.envres.2018.09.013
DO - 10.1016/j.envres.2018.09.013
M3 - Article
C2 - 30268961
AN - SCOPUS:85053940643
VL - 168
SP - 54
EP - 61
JO - Environmental Research
JF - Environmental Research
SN - 0013-9351
ER -