Abstract
BACKGROUND AND PURPOSE-: Osteoprotegerin (OPG) is a secretory glycoprotein which belongs to the tumor necrosis factor receptor family. Various mechanisms have been suggested by which calcification might alter atherosclerotic plaque stability, but the significance of this intimal calcification is controversial. High concentrations of OPG have been associated with the presence of vascular and cardiovascular diseases. This study was designed to assess the association between gene polymorphisms of the OPG gene (TNFRSF11B), the serum OPG level, and plaque stability in patients with carotid atherosclerosis. METHODS-: We studied 177 patients with internal carotid artery stenosis who underwent carotid endarterectomy and also 303 controls. Carotid endarterectomy samples removed from patients were assessed by immunohistochemistry. Concentrations of OPG were measured and gene polymorphisms were examined by polymerase chain reaction and restriction enzyme analysis and were compared, initially between patients with carotid atherosclerosis and controls, and subsequently between stable and unstable carotid plaques. RESULTS-: We found that the GG genotype of the T245G polymorphism, the CC genotype of the T950C polymorphism, and the CC genotype of the G1181C polymorphism were significantly higher in patients with carotid plaque than in controls (21.5% versus 10.9% , P
| Original language | English |
|---|---|
| Pages (from-to) | 3022-3028 |
| Number of pages | 7 |
| Journal | Stroke |
| Volume | 42 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Nov 2011 |
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Keywords
- carotid artery
- carotid atherosclerosis
- gene polymorphisms
- osteoprotegerin
- stable and unstable plaques
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
- Clinical Neurology
- Advanced and Specialised Nursing
Cite this
Assessment of the genetic effects of polymorphisms in the osteoprotegerin gene, TNFRSF11B, on serum osteoprotegerin levels and carotid plaque vulnerability. / Straface, Giuseppe; Biscetti, Federico; Pitocco, Dario; Bertoletti, Giovanni; Misuraca, Maria; Vincenzoni, Claudio; Snider, Francesco; Arena, Vincenzo; Stigliano, Egidio; Angelini, Flavia; Iuliano, Luigi; Boccia, Stefania; De Waure, Chiara; Giacchi, Francesco; Ghirlanda, Giovanni; Flex, Andrea.
In: Stroke, Vol. 42, No. 11, 11.2011, p. 3022-3028.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Assessment of the genetic effects of polymorphisms in the osteoprotegerin gene, TNFRSF11B, on serum osteoprotegerin levels and carotid plaque vulnerability
AU - Straface, Giuseppe
AU - Biscetti, Federico
AU - Pitocco, Dario
AU - Bertoletti, Giovanni
AU - Misuraca, Maria
AU - Vincenzoni, Claudio
AU - Snider, Francesco
AU - Arena, Vincenzo
AU - Stigliano, Egidio
AU - Angelini, Flavia
AU - Iuliano, Luigi
AU - Boccia, Stefania
AU - De Waure, Chiara
AU - Giacchi, Francesco
AU - Ghirlanda, Giovanni
AU - Flex, Andrea
PY - 2011/11
Y1 - 2011/11
N2 - BACKGROUND AND PURPOSE-: Osteoprotegerin (OPG) is a secretory glycoprotein which belongs to the tumor necrosis factor receptor family. Various mechanisms have been suggested by which calcification might alter atherosclerotic plaque stability, but the significance of this intimal calcification is controversial. High concentrations of OPG have been associated with the presence of vascular and cardiovascular diseases. This study was designed to assess the association between gene polymorphisms of the OPG gene (TNFRSF11B), the serum OPG level, and plaque stability in patients with carotid atherosclerosis. METHODS-: We studied 177 patients with internal carotid artery stenosis who underwent carotid endarterectomy and also 303 controls. Carotid endarterectomy samples removed from patients were assessed by immunohistochemistry. Concentrations of OPG were measured and gene polymorphisms were examined by polymerase chain reaction and restriction enzyme analysis and were compared, initially between patients with carotid atherosclerosis and controls, and subsequently between stable and unstable carotid plaques. RESULTS-: We found that the GG genotype of the T245G polymorphism, the CC genotype of the T950C polymorphism, and the CC genotype of the G1181C polymorphism were significantly higher in patients with carotid plaque than in controls (21.5% versus 10.9% , P
AB - BACKGROUND AND PURPOSE-: Osteoprotegerin (OPG) is a secretory glycoprotein which belongs to the tumor necrosis factor receptor family. Various mechanisms have been suggested by which calcification might alter atherosclerotic plaque stability, but the significance of this intimal calcification is controversial. High concentrations of OPG have been associated with the presence of vascular and cardiovascular diseases. This study was designed to assess the association between gene polymorphisms of the OPG gene (TNFRSF11B), the serum OPG level, and plaque stability in patients with carotid atherosclerosis. METHODS-: We studied 177 patients with internal carotid artery stenosis who underwent carotid endarterectomy and also 303 controls. Carotid endarterectomy samples removed from patients were assessed by immunohistochemistry. Concentrations of OPG were measured and gene polymorphisms were examined by polymerase chain reaction and restriction enzyme analysis and were compared, initially between patients with carotid atherosclerosis and controls, and subsequently between stable and unstable carotid plaques. RESULTS-: We found that the GG genotype of the T245G polymorphism, the CC genotype of the T950C polymorphism, and the CC genotype of the G1181C polymorphism were significantly higher in patients with carotid plaque than in controls (21.5% versus 10.9% , P
KW - carotid artery
KW - carotid atherosclerosis
KW - gene polymorphisms
KW - osteoprotegerin
KW - stable and unstable plaques
UR - http://www.scopus.com/inward/record.url?scp=80054996054&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80054996054&partnerID=8YFLogxK
U2 - 10.1161/STROKEAHA.111.619288
DO - 10.1161/STROKEAHA.111.619288
M3 - Article
C2 - 21903966
AN - SCOPUS:80054996054
VL - 42
SP - 3022
EP - 3028
JO - Stroke
JF - Stroke
SN - 0039-2499
IS - 11
ER -