Assessment of the genetic effects of polymorphisms in the osteoprotegerin gene, TNFRSF11B, on serum osteoprotegerin levels and carotid plaque vulnerability

Giuseppe Straface, Federico Biscetti, Dario Pitocco, Giovanni Bertoletti, Maria Misuraca, Claudio Vincenzoni, Francesco Snider, Vincenzo Arena, Egidio Stigliano, Flavia Angelini, Luigi Iuliano, Stefania Boccia, Chiara De Waure, Francesco Giacchi, Giovanni Ghirlanda, Andrea Flex

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND AND PURPOSE-: Osteoprotegerin (OPG) is a secretory glycoprotein which belongs to the tumor necrosis factor receptor family. Various mechanisms have been suggested by which calcification might alter atherosclerotic plaque stability, but the significance of this intimal calcification is controversial. High concentrations of OPG have been associated with the presence of vascular and cardiovascular diseases. This study was designed to assess the association between gene polymorphisms of the OPG gene (TNFRSF11B), the serum OPG level, and plaque stability in patients with carotid atherosclerosis. METHODS-: We studied 177 patients with internal carotid artery stenosis who underwent carotid endarterectomy and also 303 controls. Carotid endarterectomy samples removed from patients were assessed by immunohistochemistry. Concentrations of OPG were measured and gene polymorphisms were examined by polymerase chain reaction and restriction enzyme analysis and were compared, initially between patients with carotid atherosclerosis and controls, and subsequently between stable and unstable carotid plaques. RESULTS-: We found that the GG genotype of the T245G polymorphism, the CC genotype of the T950C polymorphism, and the CC genotype of the G1181C polymorphism were significantly higher in patients with carotid plaque than in controls (21.5% versus 10.9% , P

Original languageEnglish
Pages (from-to)3022-3028
Number of pages7
JournalStroke
Volume42
Issue number11
DOIs
Publication statusPublished - Nov 2011

Keywords

  • carotid artery
  • carotid atherosclerosis
  • gene polymorphisms
  • osteoprotegerin
  • stable and unstable plaques

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Advanced and Specialised Nursing

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