Assessment of the radioprotective effects of amifostine and melatonin on human lymphocytes irradiated with gamma-rays In vitro

Nevenka Kopjar, Slavica Miočić, Snježana Ramić, Mirta Milić, Tomislav Viculin

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Radioprotective effects of amifostine and melatonin as well as their ability to modulate the level of spontaneous and γ-irradiation-induced genetic changes on human peripheral blood lymphocytes were investigated using the cytokinesis-block micronucleus (CBMN) assay and sister chromatid exchange (SCE). Parallel blood samples were pre-treated with amifostine, melatonin and their combination for 30 minutes. Negative controls were also included. After the treatment with radioprotectors, one blood sample of each experimental group was exposed to γ-rays from a 60Co source. The radiation dose absorbed was 2 Gy. Our research confirmed the radioprotective effects of both chemicals in vitro, with no significant genotoxicity. Pre-treated irradiated blood samples showed a decrease in the total number of micronuclei (MN) and in the number of cells with more than one MN. They also showed significantly lower mean SCE values. This study shows that it is possible combine these radioprotectors by adjusting the doses of amifostine to achieve the best radioprotective effect with as few side effects as possible. However, further in vitro and clinical studies are needed to clarify their mechanisms of action and possible interactions.

Original languageEnglish
Pages (from-to)155-163
Number of pages9
JournalArhiv za Higijenu Rada i Toksikologiju
Volume57
Issue number2
Publication statusPublished - Jun 2006

Fingerprint

Amifostine
Lymphocytes
Gamma Rays
Melatonin
Gamma rays
Blood
Sister Chromatid Exchange
Micronucleus Tests
Cytokinesis
Dosimetry
Assays
Cell Count
Irradiation
Radiation
In Vitro Techniques
Research

Keywords

  • Ionising radiation
  • Peripheral blood
  • Radioprotectors

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Toxicology

Cite this

Assessment of the radioprotective effects of amifostine and melatonin on human lymphocytes irradiated with gamma-rays In vitro. / Kopjar, Nevenka; Miočić, Slavica; Ramić, Snježana; Milić, Mirta; Viculin, Tomislav.

In: Arhiv za Higijenu Rada i Toksikologiju, Vol. 57, No. 2, 06.2006, p. 155-163.

Research output: Contribution to journalArticle

Kopjar, Nevenka ; Miočić, Slavica ; Ramić, Snježana ; Milić, Mirta ; Viculin, Tomislav. / Assessment of the radioprotective effects of amifostine and melatonin on human lymphocytes irradiated with gamma-rays In vitro. In: Arhiv za Higijenu Rada i Toksikologiju. 2006 ; Vol. 57, No. 2. pp. 155-163.
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