Abstract
Selenium is an essential trace mineral of fundamental importance to human healthy and exerts its biological function through selenoproteins. In particular, Selenoprotein M (SELM) is located in the endoplasmic reticulum and contains the common redox motif of cysteine-X-X-selenocysteine type. It attracts great attention due to its high expression in brain and its potential roles as antioxidant, neuroprotective, and cytosolic calcium regulator. Recently, our group found SELM over-expression in human hepatocellular carcinoma (HCC) cell lines. In this report some paraffin-embedded tissues from liver biopsy of patients with hepatitis C virus (HCV)-related cirrhosis and HCC were immunohistochemically stained and SELM expression scoring was evaluated. Our results evidence for the first time an increase of SELM expression in HCC liver tissues, and its gradual expression raise associated with an increased malignancy grade. Therefore, we propose to use i) SELM as putative marker for HCC as well as ii) simple immunohistochemistry technique to distinguish between the different grades of malignancy.
| Original language | English |
|---|---|
| Pages (from-to) | 287-290 |
| Number of pages | 4 |
| Journal | European journal of histochemistry : EJH |
| Volume | 58 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Dec 1 2014 |
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Keywords
- Hepatocellular carcinoma
- Immunohistochemistry
- SELM
ASJC Scopus subject areas
- Cell Biology
- Histology
- Biophysics
Cite this
Assessment of the Selenoprotein M (SELM) over-expression on human hepatocellular carcinoma tissues by immunohistochemistry. / Guerriero, E.; Accardo, M.; Capone, F.; Colonna, G.; Castello, G.; Costantini, Susan.
In: European journal of histochemistry : EJH, Vol. 58, No. 4, 01.12.2014, p. 287-290.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Assessment of the Selenoprotein M (SELM) over-expression on human hepatocellular carcinoma tissues by immunohistochemistry
AU - Guerriero, E.
AU - Accardo, M.
AU - Capone, F.
AU - Colonna, G.
AU - Castello, G.
AU - Costantini, Susan
PY - 2014/12/1
Y1 - 2014/12/1
N2 - Selenium is an essential trace mineral of fundamental importance to human healthy and exerts its biological function through selenoproteins. In particular, Selenoprotein M (SELM) is located in the endoplasmic reticulum and contains the common redox motif of cysteine-X-X-selenocysteine type. It attracts great attention due to its high expression in brain and its potential roles as antioxidant, neuroprotective, and cytosolic calcium regulator. Recently, our group found SELM over-expression in human hepatocellular carcinoma (HCC) cell lines. In this report some paraffin-embedded tissues from liver biopsy of patients with hepatitis C virus (HCV)-related cirrhosis and HCC were immunohistochemically stained and SELM expression scoring was evaluated. Our results evidence for the first time an increase of SELM expression in HCC liver tissues, and its gradual expression raise associated with an increased malignancy grade. Therefore, we propose to use i) SELM as putative marker for HCC as well as ii) simple immunohistochemistry technique to distinguish between the different grades of malignancy.
AB - Selenium is an essential trace mineral of fundamental importance to human healthy and exerts its biological function through selenoproteins. In particular, Selenoprotein M (SELM) is located in the endoplasmic reticulum and contains the common redox motif of cysteine-X-X-selenocysteine type. It attracts great attention due to its high expression in brain and its potential roles as antioxidant, neuroprotective, and cytosolic calcium regulator. Recently, our group found SELM over-expression in human hepatocellular carcinoma (HCC) cell lines. In this report some paraffin-embedded tissues from liver biopsy of patients with hepatitis C virus (HCV)-related cirrhosis and HCC were immunohistochemically stained and SELM expression scoring was evaluated. Our results evidence for the first time an increase of SELM expression in HCC liver tissues, and its gradual expression raise associated with an increased malignancy grade. Therefore, we propose to use i) SELM as putative marker for HCC as well as ii) simple immunohistochemistry technique to distinguish between the different grades of malignancy.
KW - Hepatocellular carcinoma
KW - Immunohistochemistry
KW - SELM
UR - http://www.scopus.com/inward/record.url?scp=84923005458&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84923005458&partnerID=8YFLogxK
U2 - 10.4081/ejh.2014.2433
DO - 10.4081/ejh.2014.2433
M3 - Article
C2 - 25578973
AN - SCOPUS:84923005458
VL - 58
SP - 287
EP - 290
JO - European Journal of Histochemistry
JF - European Journal of Histochemistry
SN - 1121-760X
IS - 4
ER -