Association between BclI polymorphism in the NR3C1 gene and in vitro individual variations in lymphocyte responses to methylprednisolone

Eva Cuzzoni, Sara De Iudicibus, Fiora Bartoli, Alessandro Ventura, Giuliana Decorti

Research output: Contribution to journalArticle

Abstract

AIM To evaluate the association between the in vitro sensitivity of peripheral blood mononuclear cells (PBMCs) to methylprednisolone (MP) and the presence of genetic polymorphisms involved in glucocorticoid (GC) response. METHODS In vitro MP inhibition of the proliferation of lymphocytes stimulated with concanavalin A was determined. Non linear regression of dose-response data was performed computing the MP concentration required to reduce proliferation to 50% (IC 50). The maximum inhibition achievable at the highest MP concentration (I max) was also calculated. Moreover, the Taqman technique was used to analyze the BclI polymorphism in the NR3C1 gene and the Leu155His polymorphism in the NALP1 gene. RESULTS A significant association between the BclI mutated genotype and an increased in vitro sensitivity to GCs was observed. CONCLUSIONS The a priori evaluation of the BclI polymorphism, associated with a lymphocyte proliferation assay, could represent a useful diagnostic tool for the optimization of steroid treatment.

Original languageEnglish
Pages (from-to)651-655
Number of pages5
JournalBritish Journal of Clinical Pharmacology
Volume73
Issue number4
DOIs
Publication statusPublished - Apr 2012

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Keywords

  • BclI polymorphism
  • Glucocorticoid sensitivity
  • Lymphocyte proliferation assay

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology

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