Abstract
Context: Endocannabinoids control food intake via both central and peripheral mechanisms, and cannabinoid type-1 receptor (CB1) modulates lipogenesis in primary adipocyte cell cultures and in animal models of obesity. Objectives: We aimed to evaluate, at the population level, the frequency of a genetic polymorphism of CB1 and to study its correlation with body mass index. Design, setting and participants: Healthy subjects from a population survey carried out in southern Italy examined in 1992-1993 and older than 65 years (n=419, M=237, F=182) were divided into quintiles by body mass index (BMI). Two hundred and ten subjects were randomly sampled from the first, third and fifth quintile of BMI (BMI, respectively: 16.2-23.8=normal, 26.7-28.4=overweight, 31.6-49.7=obese) to reach a total of 70 per quintile. Their serum and white cells from the biological bank were used to measure the genotype and the blood variables for the study. Measurements: Anthropometric parameters, blood pressure, serum glucose and lipid levels were measured with standard methods; genotyping for the CB1 1359G/A polymorphism was performed using multiplex PCR. Statistical methods included χ 2 for trend, binomial and multinomial multiple logistic regression to model BMI on the genotype, controlling for potential confounders. Results: We found a clear trend of increasing relative frequency of the CB1 wild-type genotype with the increase of BMI (P=0.03) and, using a multiple logistic regression model, wild-type genotype, female gender, age, glycaemia and triglycerides were directly associated with both overweight (third quintile of BMI) and obesity (fifth quintile of BMI). Conclusions: Although performed in a limited number of subjects, our results show that the presence of the CB1 polymorphic allele was significantly associated with a lower BMI.
| Original language | English |
|---|---|
| Pages (from-to) | 908-912 |
| Number of pages | 5 |
| Journal | International Journal of Obesity |
| Volume | 31 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 2007 |
Fingerprint
Keywords
- BMI
- Cannabinoid type-1 receptor
- CB1-polymorphism
- Endocannabinoids
- Glycaemia
- Lipid profile
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Public Health, Environmental and Occupational Health
- Endocrinology
- Food Science
- Endocrinology, Diabetes and Metabolism
Cite this
Association between cannabinoid type-1 receptor polymorphism and body mass index in a southern Italian population. / Gazzerro, P.; Caruso, M. G.; Notarnicola, M.; Misciagna, G.; Guerra, V.; Laezza, C.; Bifulco, M.
In: International Journal of Obesity, Vol. 31, No. 6, 06.2007, p. 908-912.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Association between cannabinoid type-1 receptor polymorphism and body mass index in a southern Italian population
AU - Gazzerro, P.
AU - Caruso, M. G.
AU - Notarnicola, M.
AU - Misciagna, G.
AU - Guerra, V.
AU - Laezza, C.
AU - Bifulco, M.
PY - 2007/6
Y1 - 2007/6
N2 - Context: Endocannabinoids control food intake via both central and peripheral mechanisms, and cannabinoid type-1 receptor (CB1) modulates lipogenesis in primary adipocyte cell cultures and in animal models of obesity. Objectives: We aimed to evaluate, at the population level, the frequency of a genetic polymorphism of CB1 and to study its correlation with body mass index. Design, setting and participants: Healthy subjects from a population survey carried out in southern Italy examined in 1992-1993 and older than 65 years (n=419, M=237, F=182) were divided into quintiles by body mass index (BMI). Two hundred and ten subjects were randomly sampled from the first, third and fifth quintile of BMI (BMI, respectively: 16.2-23.8=normal, 26.7-28.4=overweight, 31.6-49.7=obese) to reach a total of 70 per quintile. Their serum and white cells from the biological bank were used to measure the genotype and the blood variables for the study. Measurements: Anthropometric parameters, blood pressure, serum glucose and lipid levels were measured with standard methods; genotyping for the CB1 1359G/A polymorphism was performed using multiplex PCR. Statistical methods included χ 2 for trend, binomial and multinomial multiple logistic regression to model BMI on the genotype, controlling for potential confounders. Results: We found a clear trend of increasing relative frequency of the CB1 wild-type genotype with the increase of BMI (P=0.03) and, using a multiple logistic regression model, wild-type genotype, female gender, age, glycaemia and triglycerides were directly associated with both overweight (third quintile of BMI) and obesity (fifth quintile of BMI). Conclusions: Although performed in a limited number of subjects, our results show that the presence of the CB1 polymorphic allele was significantly associated with a lower BMI.
AB - Context: Endocannabinoids control food intake via both central and peripheral mechanisms, and cannabinoid type-1 receptor (CB1) modulates lipogenesis in primary adipocyte cell cultures and in animal models of obesity. Objectives: We aimed to evaluate, at the population level, the frequency of a genetic polymorphism of CB1 and to study its correlation with body mass index. Design, setting and participants: Healthy subjects from a population survey carried out in southern Italy examined in 1992-1993 and older than 65 years (n=419, M=237, F=182) were divided into quintiles by body mass index (BMI). Two hundred and ten subjects were randomly sampled from the first, third and fifth quintile of BMI (BMI, respectively: 16.2-23.8=normal, 26.7-28.4=overweight, 31.6-49.7=obese) to reach a total of 70 per quintile. Their serum and white cells from the biological bank were used to measure the genotype and the blood variables for the study. Measurements: Anthropometric parameters, blood pressure, serum glucose and lipid levels were measured with standard methods; genotyping for the CB1 1359G/A polymorphism was performed using multiplex PCR. Statistical methods included χ 2 for trend, binomial and multinomial multiple logistic regression to model BMI on the genotype, controlling for potential confounders. Results: We found a clear trend of increasing relative frequency of the CB1 wild-type genotype with the increase of BMI (P=0.03) and, using a multiple logistic regression model, wild-type genotype, female gender, age, glycaemia and triglycerides were directly associated with both overweight (third quintile of BMI) and obesity (fifth quintile of BMI). Conclusions: Although performed in a limited number of subjects, our results show that the presence of the CB1 polymorphic allele was significantly associated with a lower BMI.
KW - BMI
KW - Cannabinoid type-1 receptor
KW - CB1-polymorphism
KW - Endocannabinoids
KW - Glycaemia
KW - Lipid profile
UR - http://www.scopus.com/inward/record.url?scp=34249671248&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34249671248&partnerID=8YFLogxK
U2 - 10.1038/sj.ijo.0803510
DO - 10.1038/sj.ijo.0803510
M3 - Article
C2 - 17160086
AN - SCOPUS:34249671248
VL - 31
SP - 908
EP - 912
JO - International Journal of Obesity
JF - International Journal of Obesity
SN - 0307-0565
IS - 6
ER -