Association between innate response to gliadin and activation of pathogenic T cells in coeliac disease

Luigi Maiuri, Carolina Ciacci, Ida Ricciardelli, Loredana Vacca, Valeria Raia, Salvatore Auricchio, Jean Picard, Mohamed Osman, Sonia Quaratino, Marco Londei

Research output: Contribution to journalArticlepeer-review

Abstract

Background: The adaptive immune system is central to the development of coeliac disease. Adaptive immune responses are, however, controlled by a preceding activation of the innate immune system. We investigated whether gliadin, a protein present in wheat flour, could activate an innate as well as an adaptive immune response in patients with coeliac disease. Methods: Duodenal biopsy samples from 42 patients with untreated coeliac disease, 37 treated patients, and 18 controls, were cultured in vitro for 3 h or 24 h, in the presence of either immunodominant gliadin epitopes (pα-2 and pα-9) or a non-immunodominant peptide (p31-43) known to induce small intestine damage in coeliac disease. We also incubated biopsy samples from nine untreated and six treated patients with a non-immunodominant peptide for 3 h, before incubation with immunodominant gliadin epitopes. Different combinations of interleukin-15 or signal transduction inhibitors were added to selected incubations. Findings: Only the non-immunodominant peptide induced rapid expression of interleukin-15, CD83, cyclo-oxygenase (COX)-2, and CD25 by CD3- cells (p=0.005 vs medium alone) and enterocyte apoptosis (p

Original languageEnglish
Pages (from-to)30-37
Number of pages8
JournalLancet
Volume362
Issue number9377
DOIs
Publication statusPublished - Jul 5 2003

ASJC Scopus subject areas

  • Medicine(all)

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