Association between methylenetetrahydrofolate reductase polymorphisms, alcohol intake and oropharyngolaryngeal carcinoma in northern Italy

Pasquale Capaccio, Francesco Ottaviani, Valeria Cuccarini, Salvatore Cenzuales, Bruno Mario Cesana, Lorenzo Pignataro

Research output: Contribution to journalArticle

Abstract

Folate metabolism dysregulation may lead to abnormal cell proliferation and predispose to carcinogenesis by inducing DNA hypomethylation. Folate pathways may be modified by polymorphisms in relevant genes, such as that for methylenetetrahydrofolate reductase (MTHFR), or by alcohol consumption. We investigated the relationship between MTHFR mutations at nucleotides C677T and A1298C, which cause reduced MTHFR enzyme activity, and susceptibility to oropharyngolaryngeal carcinoma in 65 patients and 100 controls. We isolated DNA from peripheral blood leukocytes. In oropharyngolaryngeal carcinoma cases the C677T heterozygous genotype was more frequent (p = 0.018), the allele frequency of MTHFR 677T was greater (p = 0.019) and the genotype 677TT/1298AA was more frequent (p = 0.001). A higher risk of carcinoma was found in the case of moderate drinkers with mutant MTHFR homozygosis or double heterozygosis (OR = 21.2 and OR = 9.1, respectively; p trend = 0.002), and the association was maintained for the different cancer sites (glottic, supraglottic, oropharyngeal). Our findings support the hypothesis that the interaction of alcohol intake and MTHFR polymorphisms might contribute to susceptibility to carcinogenesis of the oropharyngolaryngeal tract.

Original languageEnglish
Pages (from-to)371-376
Number of pages6
JournalJournal of Laryngology and Otology
Volume119
Issue number5
DOIs
Publication statusPublished - May 2005

Keywords

  • Alcohol Consumption
  • Carcinoma
  • Folate
  • Head and Neck Neoplasms
  • Mutation

ASJC Scopus subject areas

  • Otorhinolaryngology

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