Association between OLR1 K167N SNP and intima media thickness of the common carotid artery in the general population

Irene Marta Predazzi, Giuseppe Danilo Norata, Lucia Vecchione, Katia Garlaschelli, Francesca Amati, Liliana Grigore, Lucia Cutuli, Angela Pirillo, Simona Tramontana, Francesco Romeo, Giuseppe Novelli, Alberico Luigi Catapano

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background and Purpose: The lectin-like oxidised LDL receptor-1 (OLR1) gene encodes a scavenger receptor implicated in the pathogenesis of atherosclerosis. Although functional roles have been suggested for two variants, epidemiological studies on OLR1 have been inconsistent. Methods - We tested the association between the non-synonymous substitution K167N (rs11053646) and intima media thickness of the common carotid artery (CCA-IMT) in 2,141 samples from the Progression of Lesions in the Intima of the Carotid (PLIC) study (a prospective population-based study). Results: Significantly increased IMT was observed in male carriers of the minor C (N) allele compared to GC and GG (KN and KK) genotype. Functional analysis on macrophages suggested a decreased association to Ox-LDL in NN carriers compared to KN and KK carriers which is also associated with a reduced OLR1 mRNA expression. Macrophages from NN carriers present also a specific inflammatory gene expression pattern compared to cells from KN and KK carriers. Conclusions: These data suggest that the 167N variant of LOX-1 receptor affects the atherogenic process in the carotid artery prior to evidence of disease through an inflammatory process.

Original languageEnglish
Article numbere31086
JournalPLoS One
Volume7
Issue number2
DOIs
Publication statusPublished - Feb 9 2012

Fingerprint

Oxidized LDL Receptors
carotid arteries
Common Carotid Artery
LDL Lipoproteins
Single Nucleotide Polymorphism
Macrophages
receptors
Association reactions
Population
Scavenger Receptors
Functional analysis
Carotid Arteries
Lectins
Gene expression
macrophages
Epidemiologic Studies
Atherosclerosis
Substitution reactions
Genes
Alleles

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Association between OLR1 K167N SNP and intima media thickness of the common carotid artery in the general population. / Predazzi, Irene Marta; Norata, Giuseppe Danilo; Vecchione, Lucia; Garlaschelli, Katia; Amati, Francesca; Grigore, Liliana; Cutuli, Lucia; Pirillo, Angela; Tramontana, Simona; Romeo, Francesco; Novelli, Giuseppe; Catapano, Alberico Luigi.

In: PLoS One, Vol. 7, No. 2, e31086, 09.02.2012.

Research output: Contribution to journalArticle

Predazzi, IM, Norata, GD, Vecchione, L, Garlaschelli, K, Amati, F, Grigore, L, Cutuli, L, Pirillo, A, Tramontana, S, Romeo, F, Novelli, G & Catapano, AL 2012, 'Association between OLR1 K167N SNP and intima media thickness of the common carotid artery in the general population', PLoS One, vol. 7, no. 2, e31086. https://doi.org/10.1371/journal.pone.0031086
Predazzi, Irene Marta ; Norata, Giuseppe Danilo ; Vecchione, Lucia ; Garlaschelli, Katia ; Amati, Francesca ; Grigore, Liliana ; Cutuli, Lucia ; Pirillo, Angela ; Tramontana, Simona ; Romeo, Francesco ; Novelli, Giuseppe ; Catapano, Alberico Luigi. / Association between OLR1 K167N SNP and intima media thickness of the common carotid artery in the general population. In: PLoS One. 2012 ; Vol. 7, No. 2.
@article{cf69e87c27894eb0b970635e19227e5e,
title = "Association between OLR1 K167N SNP and intima media thickness of the common carotid artery in the general population",
abstract = "Background and Purpose: The lectin-like oxidised LDL receptor-1 (OLR1) gene encodes a scavenger receptor implicated in the pathogenesis of atherosclerosis. Although functional roles have been suggested for two variants, epidemiological studies on OLR1 have been inconsistent. Methods - We tested the association between the non-synonymous substitution K167N (rs11053646) and intima media thickness of the common carotid artery (CCA-IMT) in 2,141 samples from the Progression of Lesions in the Intima of the Carotid (PLIC) study (a prospective population-based study). Results: Significantly increased IMT was observed in male carriers of the minor C (N) allele compared to GC and GG (KN and KK) genotype. Functional analysis on macrophages suggested a decreased association to Ox-LDL in NN carriers compared to KN and KK carriers which is also associated with a reduced OLR1 mRNA expression. Macrophages from NN carriers present also a specific inflammatory gene expression pattern compared to cells from KN and KK carriers. Conclusions: These data suggest that the 167N variant of LOX-1 receptor affects the atherogenic process in the carotid artery prior to evidence of disease through an inflammatory process.",
author = "Predazzi, {Irene Marta} and Norata, {Giuseppe Danilo} and Lucia Vecchione and Katia Garlaschelli and Francesca Amati and Liliana Grigore and Lucia Cutuli and Angela Pirillo and Simona Tramontana and Francesco Romeo and Giuseppe Novelli and Catapano, {Alberico Luigi}",
year = "2012",
month = "2",
day = "9",
doi = "10.1371/journal.pone.0031086",
language = "English",
volume = "7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "2",

}

TY - JOUR

T1 - Association between OLR1 K167N SNP and intima media thickness of the common carotid artery in the general population

AU - Predazzi, Irene Marta

AU - Norata, Giuseppe Danilo

AU - Vecchione, Lucia

AU - Garlaschelli, Katia

AU - Amati, Francesca

AU - Grigore, Liliana

AU - Cutuli, Lucia

AU - Pirillo, Angela

AU - Tramontana, Simona

AU - Romeo, Francesco

AU - Novelli, Giuseppe

AU - Catapano, Alberico Luigi

PY - 2012/2/9

Y1 - 2012/2/9

N2 - Background and Purpose: The lectin-like oxidised LDL receptor-1 (OLR1) gene encodes a scavenger receptor implicated in the pathogenesis of atherosclerosis. Although functional roles have been suggested for two variants, epidemiological studies on OLR1 have been inconsistent. Methods - We tested the association between the non-synonymous substitution K167N (rs11053646) and intima media thickness of the common carotid artery (CCA-IMT) in 2,141 samples from the Progression of Lesions in the Intima of the Carotid (PLIC) study (a prospective population-based study). Results: Significantly increased IMT was observed in male carriers of the minor C (N) allele compared to GC and GG (KN and KK) genotype. Functional analysis on macrophages suggested a decreased association to Ox-LDL in NN carriers compared to KN and KK carriers which is also associated with a reduced OLR1 mRNA expression. Macrophages from NN carriers present also a specific inflammatory gene expression pattern compared to cells from KN and KK carriers. Conclusions: These data suggest that the 167N variant of LOX-1 receptor affects the atherogenic process in the carotid artery prior to evidence of disease through an inflammatory process.

AB - Background and Purpose: The lectin-like oxidised LDL receptor-1 (OLR1) gene encodes a scavenger receptor implicated in the pathogenesis of atherosclerosis. Although functional roles have been suggested for two variants, epidemiological studies on OLR1 have been inconsistent. Methods - We tested the association between the non-synonymous substitution K167N (rs11053646) and intima media thickness of the common carotid artery (CCA-IMT) in 2,141 samples from the Progression of Lesions in the Intima of the Carotid (PLIC) study (a prospective population-based study). Results: Significantly increased IMT was observed in male carriers of the minor C (N) allele compared to GC and GG (KN and KK) genotype. Functional analysis on macrophages suggested a decreased association to Ox-LDL in NN carriers compared to KN and KK carriers which is also associated with a reduced OLR1 mRNA expression. Macrophages from NN carriers present also a specific inflammatory gene expression pattern compared to cells from KN and KK carriers. Conclusions: These data suggest that the 167N variant of LOX-1 receptor affects the atherogenic process in the carotid artery prior to evidence of disease through an inflammatory process.

UR - http://www.scopus.com/inward/record.url?scp=84856775548&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84856775548&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0031086

DO - 10.1371/journal.pone.0031086

M3 - Article

VL - 7

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 2

M1 - e31086

ER -