Association between p53 Gene Mutations and Tobacco and Alcohol Exposure in Laryngeal Squamous Cell Carcinoma

Domenica Ronchetti, Cesare B. Neglia, Bruno M. Cesana, Nadia Carboni, Antonino Neri, Giancarlo Pruneri, Lorenzo Pignataro

Research output: Contribution to journalArticle

Abstract

Objectives: To analyze the relationship between p53 gene mutations, tobacco smoke, and alcohol consumption in patients with laryngeal squamous cell carcinoma. Design: We analyzed p53 gene mutations in exons 5 through 8 by polymerase chain reaction-single-strand conformation polymorphism analysis in a cohort of 84 patients with laryngeal squamous cell carcinoma. Setting: University hospital. Results: p53 gene mutations were detected in 24 (28.6%) cases (95% confidence interval, 19.3%-39.5%), and the GC to TA transversion (33%) was the most common type of mutation (95% confidence interval, 15.6%-55.3%). Most mutations mapped to the p53 DNA-binding domain, which is necessary for the physiological activity of p53 as a tumor suppressor. A statistically significant association was found between p53 mutations and exposure to tobacco smoke (P=.001), which was the only variable significantly associated with p53 mutations in a multivariate model. The association with alcohol consumption was only at a borderline level of significance (P=.065). Conclusion: Our data document that a smoking habit is the only independent variable associated with an increased risk of p53 mutations in the laryngeal mucosa.

Original languageEnglish
Pages (from-to)303-306
Number of pages4
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume130
Issue number3
DOIs
Publication statusPublished - Mar 2004

ASJC Scopus subject areas

  • Otorhinolaryngology

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