Abstract
Fifty-nine colonic adenomas and 6 hyperplastic colonic polyps were analyzed by single-strand conformation polymorphism analysis for mutations in the adenomatous polyposis coli gene (APC). Frameshifts and premature stop codons in at least one copy of APC were detected in 25 of these adenomas. Five adenomas carried 2 APC mutations. No mutations in APC were found in any of the 6 hyperplastic polyps. The detection of APC mutations increased with size and degree of dysplasia and in rectal as compared to colonic adenomas, although the association was not statistically significant. The frequency of detectable APC mutations was higher in tubulovillous and villous adenomas (10 of 13) than in tubular adenomas (15 of 45) (odds ratio, 6.67; 95% confidence limits, 1.39-41.83; P = 0.005). The significance of the association between the detection of APC mutations and a villous architecture was confirmed in multivariate analysis (relative risk, 6.67; 95% confidence limits, 1.54- 28.8; P = 0.005). In conclusion, APC mutation plays a role in adenoma progression; its frequency is significantly higher in lesions with a more villous morphology.
| Original language | English |
|---|---|
| Pages (from-to) | 3553-3556 |
| Number of pages | 4 |
| Journal | Cancer Research |
| Volume | 54 |
| Issue number | 13 |
| Publication status | Published - Jul 1 1994 |
Fingerprint
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Association of APC gene mutations and histological characteristics of colorectal adenomas. / De Benedetti, Laura; Sciallero, Stefania; Gismondi, Viviana; James, Rosella; Bafico, Anna; Biticchi, Roberta; Masetti, Emiliana; Bonelli, Luigina; Heouaine, Abdelhamid; Picasso, Massimo; Groden, Joanna; Robertson, Margaret; Risio, Mauro; Caprilli, Renzo; Bruzzi, Paolo; White, Raymond L.; Aste, Hugo; Santi, Leonardo; Varesco, Liliana; Ferrara, Giovannni Battista.
In: Cancer Research, Vol. 54, No. 13, 01.07.1994, p. 3553-3556.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Association of APC gene mutations and histological characteristics of colorectal adenomas
AU - De Benedetti, Laura
AU - Sciallero, Stefania
AU - Gismondi, Viviana
AU - James, Rosella
AU - Bafico, Anna
AU - Biticchi, Roberta
AU - Masetti, Emiliana
AU - Bonelli, Luigina
AU - Heouaine, Abdelhamid
AU - Picasso, Massimo
AU - Groden, Joanna
AU - Robertson, Margaret
AU - Risio, Mauro
AU - Caprilli, Renzo
AU - Bruzzi, Paolo
AU - White, Raymond L.
AU - Aste, Hugo
AU - Santi, Leonardo
AU - Varesco, Liliana
AU - Ferrara, Giovannni Battista
PY - 1994/7/1
Y1 - 1994/7/1
N2 - Fifty-nine colonic adenomas and 6 hyperplastic colonic polyps were analyzed by single-strand conformation polymorphism analysis for mutations in the adenomatous polyposis coli gene (APC). Frameshifts and premature stop codons in at least one copy of APC were detected in 25 of these adenomas. Five adenomas carried 2 APC mutations. No mutations in APC were found in any of the 6 hyperplastic polyps. The detection of APC mutations increased with size and degree of dysplasia and in rectal as compared to colonic adenomas, although the association was not statistically significant. The frequency of detectable APC mutations was higher in tubulovillous and villous adenomas (10 of 13) than in tubular adenomas (15 of 45) (odds ratio, 6.67; 95% confidence limits, 1.39-41.83; P = 0.005). The significance of the association between the detection of APC mutations and a villous architecture was confirmed in multivariate analysis (relative risk, 6.67; 95% confidence limits, 1.54- 28.8; P = 0.005). In conclusion, APC mutation plays a role in adenoma progression; its frequency is significantly higher in lesions with a more villous morphology.
AB - Fifty-nine colonic adenomas and 6 hyperplastic colonic polyps were analyzed by single-strand conformation polymorphism analysis for mutations in the adenomatous polyposis coli gene (APC). Frameshifts and premature stop codons in at least one copy of APC were detected in 25 of these adenomas. Five adenomas carried 2 APC mutations. No mutations in APC were found in any of the 6 hyperplastic polyps. The detection of APC mutations increased with size and degree of dysplasia and in rectal as compared to colonic adenomas, although the association was not statistically significant. The frequency of detectable APC mutations was higher in tubulovillous and villous adenomas (10 of 13) than in tubular adenomas (15 of 45) (odds ratio, 6.67; 95% confidence limits, 1.39-41.83; P = 0.005). The significance of the association between the detection of APC mutations and a villous architecture was confirmed in multivariate analysis (relative risk, 6.67; 95% confidence limits, 1.54- 28.8; P = 0.005). In conclusion, APC mutation plays a role in adenoma progression; its frequency is significantly higher in lesions with a more villous morphology.
UR - http://www.scopus.com/inward/record.url?scp=0028359236&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028359236&partnerID=8YFLogxK
M3 - Article
C2 - 8012980
AN - SCOPUS:0028359236
VL - 54
SP - 3553
EP - 3556
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0008-5472
IS - 13
ER -