Association of chromatin proteins high mobility group box (HMGB) 1 and HMGB2 with mitotic chromosomes

Coralie Pallier, Paola Scaffidi, Stéphanie Chopineau-Proust, Alessandra Agresti, Patrice Nordmann, Marco E. Bianchi, Vincent Marechal

Research output: Contribution to journalArticle

Abstract

High mobility group box (HMGB) 1 and 2 are two abundant nonhistone nuclear proteins that have been found in association with chromatin. Previous studies based on immunofluorescence analysis indicated that HMGB1 dissociates from chromosomes during mitosis. In the present work, HMGB1 and 2 subcellular localization was reinvestigated in living cells by using enhanced green fluorescent protein- and Discosome sp. red fluorescent protein-tagged proteins. Contrary to previous reports, HMGB1 and 2 were shown to be present under two forms in mitotic cells, i.e., free and associated with the condensed chromatin, which rapidly exchange. A detailed analysis of HMGB2 interaction with mitotic chromosomes indicated that two sites encompassing HMG-box A and B are responsible for binding. Importantly, this interaction was rapidly inactivated when cells were permeabilized or exposed to chemical fixatives that are widely used in immunodetection techniques. A comparable behavior was also observed for two proteins of the HMG-nucleosome binding (HMGN) group, namely, HMGN1 and HMGN2.

Original languageEnglish
Pages (from-to)3414-3426
Number of pages13
JournalMolecular Biology of the Cell
Volume14
Issue number8
DOIs
Publication statusPublished - Aug 1 2003

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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