Abstract
Background. We have studied the effects of interferon (IFN)-γ allelic variations on expression levels of pro- and anti-inflammatory cytokines and on long-term inflammatory status in haemodialysis patients. Methods. Genotyping was performed in 123 patients for single nucleotide polymorphisms in the first intron of the IFN-γ gene (+874 T/A). They were prospectively followed for 2 years. Cytokine mRNA levels in whole blood cells (detected by real time (RT)-PCR technique) and serum C-reactive protein (CRP) concentrations were compared in patient groups with different IFN-γ genotypes. Serum CRP was evaluated every month and inflammatory state was defined as percent of abnormal values (above 5 mg/l) over total determinations. Of the total, 102 patients survived and completed 24±1 monthly CRP determinations. The IFN-γ ±874 A/A, 'low-producer' genotype was associated with decreased (P
| Original language | English |
|---|---|
| Pages (from-to) | 1317-1322 |
| Number of pages | 6 |
| Journal | Nephrology Dialysis Transplantation |
| Volume | 21 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 2006 |
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Keywords
- C-reactive protein
- Chronic renal failure
- Haemodialysis
- Inflammation
- Interferon-gamma
- Polymorphisms
ASJC Scopus subject areas
- Nephrology
- Transplantation
Cite this
Association of interferon-γ +874A polymorphism with reduced long-term inflammatory response in haemodialysis patients. / Biolo, Gianni; Amoroso, Antonio; Savoldi, Silvana; Bosutti, Alessandra; Martone, Massimiliano; Pirulli, Doroti; Bianco, Francesco; Ulivi, Sheila; Bertok, Sara; Artero, Mary; Barazzoni, Rocco; Zanetti, Michela; Grassi, Gabriele; Guarnieri, Gianfranco; Panzetta, Giovanni.
In: Nephrology Dialysis Transplantation, Vol. 21, No. 5, 05.2006, p. 1317-1322.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Association of interferon-γ +874A polymorphism with reduced long-term inflammatory response in haemodialysis patients
AU - Biolo, Gianni
AU - Amoroso, Antonio
AU - Savoldi, Silvana
AU - Bosutti, Alessandra
AU - Martone, Massimiliano
AU - Pirulli, Doroti
AU - Bianco, Francesco
AU - Ulivi, Sheila
AU - Bertok, Sara
AU - Artero, Mary
AU - Barazzoni, Rocco
AU - Zanetti, Michela
AU - Grassi, Gabriele
AU - Guarnieri, Gianfranco
AU - Panzetta, Giovanni
PY - 2006/5
Y1 - 2006/5
N2 - Background. We have studied the effects of interferon (IFN)-γ allelic variations on expression levels of pro- and anti-inflammatory cytokines and on long-term inflammatory status in haemodialysis patients. Methods. Genotyping was performed in 123 patients for single nucleotide polymorphisms in the first intron of the IFN-γ gene (+874 T/A). They were prospectively followed for 2 years. Cytokine mRNA levels in whole blood cells (detected by real time (RT)-PCR technique) and serum C-reactive protein (CRP) concentrations were compared in patient groups with different IFN-γ genotypes. Serum CRP was evaluated every month and inflammatory state was defined as percent of abnormal values (above 5 mg/l) over total determinations. Of the total, 102 patients survived and completed 24±1 monthly CRP determinations. The IFN-γ ±874 A/A, 'low-producer' genotype was associated with decreased (P
AB - Background. We have studied the effects of interferon (IFN)-γ allelic variations on expression levels of pro- and anti-inflammatory cytokines and on long-term inflammatory status in haemodialysis patients. Methods. Genotyping was performed in 123 patients for single nucleotide polymorphisms in the first intron of the IFN-γ gene (+874 T/A). They were prospectively followed for 2 years. Cytokine mRNA levels in whole blood cells (detected by real time (RT)-PCR technique) and serum C-reactive protein (CRP) concentrations were compared in patient groups with different IFN-γ genotypes. Serum CRP was evaluated every month and inflammatory state was defined as percent of abnormal values (above 5 mg/l) over total determinations. Of the total, 102 patients survived and completed 24±1 monthly CRP determinations. The IFN-γ ±874 A/A, 'low-producer' genotype was associated with decreased (P
KW - C-reactive protein
KW - Chronic renal failure
KW - Haemodialysis
KW - Inflammation
KW - Interferon-gamma
KW - Polymorphisms
UR - http://www.scopus.com/inward/record.url?scp=33646196729&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646196729&partnerID=8YFLogxK
U2 - 10.1093/ndt/gfk033
DO - 10.1093/ndt/gfk033
M3 - Article
C2 - 16410273
AN - SCOPUS:33646196729
VL - 21
SP - 1317
EP - 1322
JO - Nephrology Dialysis Transplantation
JF - Nephrology Dialysis Transplantation
SN - 0931-0509
IS - 5
ER -