Association of lung adenocarcinoma clinical stage with gene expression pattern in noninvolved lung tissue

Elisa Frullanti, Francesca Colombo, Felicia S. Falvella, Antonella Galvan, Sara Noci, Loris De Cecco, Matteo Incarbone, Marco Alloisio, Luigi Santambrogio, Mario Nosotti, Davide Tosi, Ugo Pastorino, Tommaso A. Dragani

Research output: Contribution to journalArticlepeer-review

Abstract

Associations between clinical outcome of cancer patients and the gene expression signature in primary tumors at time of diagnosis have been reported. To test whether gene expression patterns in noninvolved lung tissue might correlate with clinical stage in lung adenocarcinoma (ADCA) patients, we compared the transcriptome of noninvolved lung samples from 60 ADCA smoker patients of clinical stage I versus 60 patients with stage >I. Quantitative PCR of 10 genes with the most significant differential expression confirmed the statistical association with clinical stage in eight genes, six of which were downregulated in high-stage patients. Five of these six genes were also downregulated in lung ADCA tissue as compared to noninvolved tissue. Studies in vitro indicated that four of the genes (SLC14A1, SMAD6, TMEM100 and TXNIP) inhibited colony formation of lung cancer cell lines transfected to overexpress the genes, suggesting their potential tumor-suppressor activity. Our findings suggest that individual variations in the transcriptional profile of noninvolved lung tissue may reflect the lung ADCA patient's predisposition to tumor aggressiveness.

Original languageEnglish
JournalInternational Journal of Cancer
Volume131
Issue number5
DOIs
Publication statusPublished - Sep 1 2012

Keywords

  • Gene expression profile
  • Lung adenocarcinoma
  • Transcriptome
  • Tumor progression

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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