Association of p53 gene and protein alterations with metastases in colorectal cancer

R. Bertorelle, G. Esposito, A. Del Mistro, C. Belluco, D. Nitti, M. Lise, L. Chieco-Bianchi

Research output: Contribution to journalArticle

Abstract

Using monoclonal antibody PAb 1801, p53 protein was detected in the neoplastic cells of 39 (46.9%) of 83 colorectal carcinomas studied. Patients with p53+ tumors showed a higher incidence of lymph node and liver metastases (p = 0.035); in patients whose tumors were located in the rectosigmoid, p53 expression also correlated with a more advanced stage according to Dukes' classification (p = 0.015) as well as nodal (p = 0.006) and liver (p = 0.019)metastases. Following amplification of exons 5 to 8 of the p53 gene by means of the polymerase chain reaction technique, single-strand conformation polymorphism analysis disclosed an anomalous migration pattern in 23 of the 39 p53+ tumors and in four of the 35 p53-tumors analyzed. Sequence analysis showed G:C → A:T transitions in 63.6%, G:C → T:A and G:C → C:G transversions in 18.2%, deletions and insertions in 13.6%, and A:T → G:C transitions in 4.6% of the cases. Loss of heterozygosity was studied in the DNA of 79 patients; allelic loss was found in 29 (49.1%) of the 59 informative patients. Loss of heterozygosity was correlated with p53 overexpression (p = 0.0002) as well as with the presence of mutations as detected by single strand conformation polymorphism analysis (p = 0.0024).

Original languageEnglish
Pages (from-to)463-471
Number of pages9
JournalAmerican Journal of Surgical Pathology
Volume19
Issue number4
Publication statusPublished - 1995

Keywords

  • Colorectal carcinoma
  • Metastases
  • p53

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine

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