Abstract
Prostaglandins, especially prostaglandin E synthetase (PGE2), influence carcinogenesis by promoting cell proliferation, inhibiting apoptosis, stimulating angiogenesis and mediating immune suppression. Cyclooxygenase-2, coded by the PTGS2 gene, is the key enzyme in the production of prostaglandins. In melanoma, Cox-2 is over expressed in primary malignant melanoma (MM) and in their corresponding metastases. Polymorphisms in the promoter region of PTGS2 gene can modulate gene expression and could modify individual susceptibility to MM. Two hundred and forty melanoma patients and 342 controls were genotyped for polymorphisms -765G>C (rs20417) and -1195A>G (rs689466). Allele -765C frequency was significantly higher in melanoma patients. No allele frequency differences for -1195A>G polymorphism were observed. Haplotype analysis revealed that the haplotypes carrying the minor alleles were associated to a higher risk of melanoma (P = 0.02). Expression analysis showed that allele -765C is associated to a higher gene expression and could represent a risk allele by affecting the functionality of the promoter.
| Original language | English |
|---|---|
| Pages (from-to) | 766-768 |
| Number of pages | 3 |
| Journal | Experimental Dermatology |
| Volume | 23 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 1 2014 |
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Keywords
- Expression analysis
- Malignant melanoma
- Promoter polymorphisms
- PTGS2
ASJC Scopus subject areas
- Dermatology
- Molecular Biology
- Biochemistry
Cite this
Association of promoter polymorphism -765G>C in the PTGS2 gene with malignant melanoma in Italian patients and its correlation to gene expression in dermal fibroblasts. / Gomez-Lira, Macarena; Ferronato, Silvia; Malerba, Giovanni; Santinami, Mario; Maurichi, Andrea; Sangalli, Antonella; Turco, Alberto; Perego, Paola; Rodolfo, Monica.
In: Experimental Dermatology, Vol. 23, No. 10, 01.10.2014, p. 766-768.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Association of promoter polymorphism -765G>C in the PTGS2 gene with malignant melanoma in Italian patients and its correlation to gene expression in dermal fibroblasts
AU - Gomez-Lira, Macarena
AU - Ferronato, Silvia
AU - Malerba, Giovanni
AU - Santinami, Mario
AU - Maurichi, Andrea
AU - Sangalli, Antonella
AU - Turco, Alberto
AU - Perego, Paola
AU - Rodolfo, Monica
PY - 2014/10/1
Y1 - 2014/10/1
N2 - Prostaglandins, especially prostaglandin E synthetase (PGE2), influence carcinogenesis by promoting cell proliferation, inhibiting apoptosis, stimulating angiogenesis and mediating immune suppression. Cyclooxygenase-2, coded by the PTGS2 gene, is the key enzyme in the production of prostaglandins. In melanoma, Cox-2 is over expressed in primary malignant melanoma (MM) and in their corresponding metastases. Polymorphisms in the promoter region of PTGS2 gene can modulate gene expression and could modify individual susceptibility to MM. Two hundred and forty melanoma patients and 342 controls were genotyped for polymorphisms -765G>C (rs20417) and -1195A>G (rs689466). Allele -765C frequency was significantly higher in melanoma patients. No allele frequency differences for -1195A>G polymorphism were observed. Haplotype analysis revealed that the haplotypes carrying the minor alleles were associated to a higher risk of melanoma (P = 0.02). Expression analysis showed that allele -765C is associated to a higher gene expression and could represent a risk allele by affecting the functionality of the promoter.
AB - Prostaglandins, especially prostaglandin E synthetase (PGE2), influence carcinogenesis by promoting cell proliferation, inhibiting apoptosis, stimulating angiogenesis and mediating immune suppression. Cyclooxygenase-2, coded by the PTGS2 gene, is the key enzyme in the production of prostaglandins. In melanoma, Cox-2 is over expressed in primary malignant melanoma (MM) and in their corresponding metastases. Polymorphisms in the promoter region of PTGS2 gene can modulate gene expression and could modify individual susceptibility to MM. Two hundred and forty melanoma patients and 342 controls were genotyped for polymorphisms -765G>C (rs20417) and -1195A>G (rs689466). Allele -765C frequency was significantly higher in melanoma patients. No allele frequency differences for -1195A>G polymorphism were observed. Haplotype analysis revealed that the haplotypes carrying the minor alleles were associated to a higher risk of melanoma (P = 0.02). Expression analysis showed that allele -765C is associated to a higher gene expression and could represent a risk allele by affecting the functionality of the promoter.
KW - Expression analysis
KW - Malignant melanoma
KW - Promoter polymorphisms
KW - PTGS2
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UR - http://www.scopus.com/inward/citedby.url?scp=84907858460&partnerID=8YFLogxK
U2 - 10.1111/exd.12522
DO - 10.1111/exd.12522
M3 - Article
C2 - 25060715
AN - SCOPUS:84907858460
VL - 23
SP - 766
EP - 768
JO - Experimental Dermatology
JF - Experimental Dermatology
SN - 0906-6705
IS - 10
ER -