Astrocytes are required for the oscillatory activity in cultured hippocampal neurons

Claudia Verderio, Alberto Bacci, Silvia Coco, Elena Pravettoni, Guido Fumagalli, Michela Matteoli

Research output: Contribution to journalArticlepeer-review

Abstract

Synchronous oscillations of intracellular calcium concentration ([Ca2+](i)) and of membrane potential occurred in a limited population of glutamatergic hippocampal neurons grown in primary cultures. The oscillatory activity occurred in synaptically connected cells only when they were in the presence of astrocytes. Microcultures containing only one or a few neurons also displayed oscillatory activity, provided that glial cells participated in the network. The glutamate-transporter inhibitors L-trans-pyrrolidine-2,4-dicarboxylic acid (PDC) and dihydrokainate, which produce an accumulation of glutamate in the synaptic microenvironment, impaired the oscillatory activity. Moreover, in neurons not spontaneously oscillating, though in the presence of astrocytes, oscillations were induced by exogenous L-glutamate, but not by the stereoisomer D-glutamate, which is not taken up by glutamate transporters. These data demonstrate that astrocytes are essential for neuronal oscillatory activity and provide evidence that removal of glutamate from the synaptic environment is one of the major mechanisms by which glial cells allow the repetitive excitation of the postsynaptic cell.

Original languageEnglish
Pages (from-to)2793-2800
Number of pages8
JournalEuropean Journal of Neuroscience
Volume11
Issue number8
DOIs
Publication statusPublished - Aug 1999

Keywords

  • Astrocytes
  • Glutamate transporters
  • Oscillations
  • Rat hippocampal neurons
  • Synaptic transmission

ASJC Scopus subject areas

  • Neuroscience(all)

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