AT1 angiotensin II receptor mediates intracellular calcium mobilization in normal and cancerous breast cells in primary culture

S. Greco, M. G. Elia, A. Muscella, C. Storelli, S. Marsigliante

Research output: Contribution to journalArticle

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Abstract

Angiotensin II (Ang II) increases intracellular calcium concentration ([Ca2+]i) in both normal and cancerous human breast cells in primary culture. Maximal [Ca2+]i increase is obtained using 100 nM Ang II in both cell types; in cancerous breast cells, [Ca2+]i increase (Δ[Ca2+]i) is 135 ± 10 nM, while in normal breast cells it reaches 65 ± 5 nM (P <0.0001). In both cell types, Ang II evokes a Ca2+ transient peak mediated by thapsigargin (TG) sensitive stores; neither Ca2+ entry through L-type membrane channels or capacitative Ca2+ entry are involved. Type I Ang II receptor subtype (AT1) mediates Ang II-dependent [Ca2+]i increase, since losartan, an AT1 inhibitor, blunted [Ca2+]i increase induced by Ang II in a dose-dependent manner, while CGP 4221A, an AT2 inhibitor, does not. Phospholipase C (PLC) is involved in this signaling mechanism, as U73122, a PLC inhibitor, decreases Ang II-dependent [Ca2+]i transient peak in a dose-dependent mode. Thus, the present study provides new information about Ca2+ signaling pathways mediated through AT1 in breast cells in wich no data were yet available.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalCell Calcium
Volume32
Issue number1
DOIs
Publication statusPublished - Jul 2002

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Angiotensin Receptors
Primary Cell Culture
Angiotensin II
Breast
Calcium
Type C Phospholipases
Thapsigargin
Losartan
Ion Channels

ASJC Scopus subject areas

  • Cell Biology
  • Endocrinology

Cite this

AT1 angiotensin II receptor mediates intracellular calcium mobilization in normal and cancerous breast cells in primary culture. / Greco, S.; Elia, M. G.; Muscella, A.; Storelli, C.; Marsigliante, S.

In: Cell Calcium, Vol. 32, No. 1, 07.2002, p. 1-10.

Research output: Contribution to journalArticle

Greco, S. ; Elia, M. G. ; Muscella, A. ; Storelli, C. ; Marsigliante, S. / AT1 angiotensin II receptor mediates intracellular calcium mobilization in normal and cancerous breast cells in primary culture. In: Cell Calcium. 2002 ; Vol. 32, No. 1. pp. 1-10.
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